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Infection and Immunity, March 2003, p. 1109-1115, Vol. 71, No. 3
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.3.1109-1115.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Enteric Salmonella Infection Inhibits Paneth Cell Antimicrobial Peptide Expression

Nita H. Salzman,1,2* Margaret M. Chou,3 Hendrik de Jong,2 Lide Liu,4 Edith M. Porter,4,5 and Yvonne Paterson2

Department of Pediatrics, Division of Gastroenterology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226,1 Departments of Microbiology,2 Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104,3 Department of Medicine, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, California 90095,4 Department of Biological Sciences, California State University Los Angeles, Los Angeles, California 900325

Received 17 October 2002/ Returned for modification 26 November 2002/ Accepted 12 December 2002

Paneth cells, highly secretory epithelial cells found at the bases of small intestinal crypts, release a variety of microbicidal molecules, including {alpha}-defensins and lysozyme. The secretion of antimicrobials by Paneth cells is thought to be important in mucosal host defense against invasion by enteric pathogens. We explored whether enteric pathogens can interfere with this arm of defense. We found that oral inoculation of mice with wild-type Salmonella enterica serovar Typhimurium decreases the expression of {alpha}-defensins (called cryptdins in mice) and lysozyme. Oral inoculation with Salmonella serovar Typhimurium strains that are heat killed, lack the PhoP regulon, and lack the SPI1 type III secretion system or with Listeria monocytogenes does not have this effect. Salmonella may gain a specific survival advantage in the intestinal lumen by decreasing the expression of microbicidal peptides in Paneth cells through direct interactions between Salmonella and the small intestinal epithelium.

* Corresponding author. Mailing address: Medical College of Wisconsin, Department of Pediatrics, 8701 Watertown Plank Rd., Milwaukee, WI 53226. Phone: (414) 456-4244. Fax: (414) 456-6535. E-mail: nsalzman{at}mcw.edu.

Editor: J. D. Clements

Infection and Immunity, March 2003, p. 1109-1115, Vol. 71, No. 3
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.3.1109-1115.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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