This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gomes, J. A. S.
Right arrow Articles by Correa-Oliveira, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gomes, J. A. S.
Right arrow Articles by Correa-Oliveira, R.

 Previous Article  |  Next Article 

Infection and Immunity, March 2003, p. 1185-1193, Vol. 71, No. 3
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.3.1185-1193.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Evidence that Development of Severe Cardiomyopathy in Human Chagas' Disease Is Due to a Th1-Specific Immune Response

J. A. S. Gomes,1,2 L. M. G. Bahia-Oliveira,3 M. O. C. Rocha,4 O. A. Martins-Filho,2 G. Gazzinelli,2 and R. Correa-Oliveira2*

Departamento de Bioquímica e Imunologia,1 Faculdade de Medicina, Curso de Pós-graduação em Medicina Tropical, Universidade Federal de Minas Gerais,4 Centro de Pesquisas René Rachou, Fiocruz, Belo Horizonte, Minas Gerais,2 Laboratório de Biologia do Reconhecer, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, Rio de Janeiro, Brazil3

Received 11 June 2002/ Returned for modification 27 August 2002/ Accepted 3 December 2002

The role of interleukin 10 (IL-10) and gamma interferon (IFN-{gamma}) on the development of pathology in human Chagas' disease was investigated. Two categories of patients, low and high producers of IFN-{gamma}, were identified based on the levels of secretion of this cytokine in the supernatant of peripheral blood mononuclear cell (PBMC) cultures. Eighty-three percent of the patients presenting with cardiac disease (CARD) of different degrees and 59% of the patients with the indeterminate form of disease (IND) were identified as high IFN-{gamma} producers. PBMC from IND patients classified as low IFN-{gamma} producers secreted significantly higher amounts of IL-10 than did those from other groups. Flow cytometry analysis demonstrated that in PBMC from the IND group, the majority of the IL-10-producing cells were monocytes (CD14High+ cells), whereas in the CARD group, the major sources of IFN-{gamma} were T lymphocytes (CD3+ CD4+ cells). These results suggest an association between the production of IFN-{gamma} by CD3+ CD4+ cells and morbidity in Chagas' disease, whereas the production of IL-10 by macrophages/monocytes leads to regulation of the immune response in IND patients. We hypothesize that an exacerbated production of IFN-{gamma} against Trypanosoma cruzi antigens favors the development of a strong Th1 response in CARD patients, which leads to progression of heart disease.

* Corresponding author. Mailing address: Centro de Pesquisas René Rachou/FIOCRUZ, Laboratório de Imunologia Celular e Molecular, Av. Augusto de Lima 1715, 30190-002, Belo Horizonte, MG, Brazil. Phone: 55 31 32953566. Fax: 55 31 32953115. E-mail: correa{at}cpqrr.fiocruz.br.

Editor: J. M. Mansfield

Infection and Immunity, March 2003, p. 1185-1193, Vol. 71, No. 3
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.3.1185-1193.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Dhiman, M., Estrada-Franco, J. G., Pando, J. M., Ramirez-Aguilar, F. J., Spratt, H., Vazquez-Corzo, S., Perez-Molina, G., Gallegos-Sandoval, R., Moreno, R., Garg, N. J. (2009). Increased Myeloperoxidase Activity and Protein Nitration Are Indicators of Inflammation in Patients with Chagas' Disease. CVI 16: 660-666 [Abstract] [Full Text]  
  • Reifenberg, K., Lehr, H.-A., Torzewski, M., Steige, G., Wiese, E., Kupper, I., Becker, C., Ott, S., Nusser, P., Yamamura, K.-I., Rechtsteiner, G., Warger, T., Pautz, A., Kleinert, H., Schmidt, A., Pieske, B., Wenzel, P., Munzel, T., Lohler, J. (2007). Interferon-{gamma} Induces Chronic Active Myocarditis and Cardiomyopathy in Transgenic Mice. Am. J. Pathol. 171: 463-472 [Abstract] [Full Text]  
  • Souza, P. E. A., Rocha, M. O. C., Menezes, C. A. S., Coelho, J. S., Chaves, A. C. L., Gollob, K. J., Dutra, W. O. (2007). Trypanosoma cruzi Infection Induces Differential Modulation of Costimulatory Molecules and Cytokines by Monocytes and T Cells from Patients with Indeterminate and Cardiac Chagas' Disease. Infect. Immun. 75: 1886-1894 [Abstract] [Full Text]  
  • Marin-Neto, J. A., Cunha-Neto, E., Maciel, B. C., Simoes, M. V. (2007). Pathogenesis of Chronic Chagas Heart Disease. Circulation 115: 1109-1123 [Abstract] [Full Text]  
  • HECHT, M. M., BUSSACOS, A. C., LOZZI, S. P., SANTANA, J. M., TEIXEIRA, A. R.L. (2006). TRIATOMA INFESTANS CHOOSES TO FEED UPON IMMUNE PREY. Am J Trop Med Hyg 75: 893-900 [Abstract] [Full Text]  
  • Sardinha, L. R., Elias, R. M., Mosca, T., Bastos, K. R. B., Marinho, C. R. F., D'Imperio Lima, M. R., Alvarez, J. M. (2006). Contribution of NK, NK T, {gamma}{delta} T, and {alpha}{beta} T Cells to the Gamma Interferon Response Required for Liver Protection against Trypanosoma cruzi. Infect. Immun. 74: 2031-2042 [Abstract] [Full Text]  
  • Gomes, J. A. S., Bahia-Oliveira, L. M. G., Rocha, M. O. C., Busek, S. C. U., Teixeira, M. M., Silva, J. S., Correa-Oliveira, R. (2005). Type 1 Chemokine Receptor Expression in Chagas' Disease Correlates with Morbidity in Cardiac Patients. Infect. Immun. 73: 7960-7966 [Abstract] [Full Text]  
  • Cunha-Neto, E., Dzau, V. J., Allen, P. D., Stamatiou, D., Benvenutti, L., Higuchi, M. L., Koyama, N. S., Silva, J. S., Kalil, J., Liew, C.-C. (2005). Cardiac Gene Expression Profiling Provides Evidence for Cytokinopathy as a Molecular Mechanism in Chagas' Disease Cardiomyopathy. Am. J. Pathol. 167: 305-313 [Abstract] [Full Text]  
  • Marino, A. P. M.P., da Silva, A., dos Santos, P., Pinto, L. M. d. O., Gazzinelli, R. T., Teixeira, M. M., Lannes-Vieira, J. (2004). Regulated on Activation, Normal T Cell Expressed and Secreted (RANTES) Antagonist (Met-RANTES) Controls the Early Phase of Trypanosoma cruzi-Elicited Myocarditis. Circulation 110: 1443-1449 [Abstract] [Full Text]  
  • Dumonteil, E., Escobedo-Ortegon, J., Reyes-Rodriguez, N., Arjona-Torres, A., Ramirez-Sierra, M. J. (2004). Immunotherapy of Trypanosoma cruzi Infection with DNA Vaccines in Mice. Infect. Immun. 72: 46-53 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»