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Infection and Immunity, March 2003, p. 1217-1224, Vol. 71, No. 3
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.3.1217-1224.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Expression of Truncated Internalin A Is Involved in Impaired Internalization of Some Listeria monocytogenes Isolates Carried Asymptomatically by Humans

Maïwenn Olier,1 Fabrice Pierre,1,{dagger} Sandrine Rousseaux,1 Jean-Paul Lemaître,1* André Rousset,1,2 Pascal Piveteau,1 and Jean Guzzo1

Laboratoire de Microbiologie Equipe PG2MA, UMR INRA 1082, ENSBANA, 21000 Dijon,1 Service Biologie, Centre Hospitalier Général de Beaune, 21200 Beaune, France2

Received 29 July 2002/ Returned for modification 24 September 2002/ Accepted 8 November 2002

Fourteen human carriage Listeria monocytogenes isolates were compared to sporadic and epidemic-associated human strains in order to ascertain the pathogenic behavior of these unrecognized asymptomatic strains. Experimental infection of 14-day-old chick embryos revealed that the majority of the carriage strains were attenuated for virulence. Of the 10 attenuated carriage strains, 5 were affected in their invasion capacities in vitro. Western blot analysis with antibody directed against InlA, the surface protein implicated in the internalization in host cells, allowed correlation between the ability of the carriage strains to enter Caco-2 cells and InlA expression. Indeed, these five carriage strains produced truncated forms of InlA. Four of the five truncated forms of InlA had an apparent molecular mass of 47 kDa. In order to assess the existence of a genetic lineage, partial sequences of inlA gene of these four strains were compared and revealed that they had a high degree of sequence conservation at the gene (99.86%) and amino acid (100%) levels. Comparison of their nucleotide sequences with that of the corresponding segment of inlA from EGD-e and Scott A strains, taken as epidemic references, showed more divergence. Taken together, these observations suggest the presence of specific traits that characterize L. monocytogenes strains isolated during asymptomatic carriage. Some of these traits could provide some explanations about the determinants that make them unable to cause systemic human infection.

* Corresponding author. Mailing address: Laboratoire de Microbiologie Equipe PG2MA, UMR INRA 1082, ENSBANA, 1 Esplanade Erasme, 21000 Dijon, France. Phone: 33-3-80-39-66-79. Fax: 33-3-80-39-39-55. E-mail: jplemait{at}u-bourgogne.fr.

Editor: A. D. O'Brien

{dagger} Present address: Equipe Aliment et Cancer UMR INRA-ENVT 1089, Toulouse, France.

Infection and Immunity, March 2003, p. 1217-1224, Vol. 71, No. 3
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.3.1217-1224.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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