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Infection and Immunity, March 2003, p. 1288-1294, Vol. 71, No. 3
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.3.1288-1294.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Human Immune Response to Outer Membrane Protein CD of Moraxella catarrhalis in Adults with Chronic Obstructive Pulmonary Disease

Timothy F. Murphy,1,2,3* Charmaine Kirkham,1 Dai-Fang Liu,4 and Sanjay Sethi3,5

Divisions of Infectious Diseases,1 Pulmonary and Critical Care Medicine, Department of Medicine,5 Department of Microbiology, University at Buffalo, The State University of New York,2 Veterans Affairs Western New York Healthcare System, Buffalo,3 Wyeth Vaccines Research, West Henrietta, New York4

Received 19 September 2002/ Returned for modification 22 October 2002/ Accepted 29 November 2002

Moraxella catarrhalis is a common cause of lower respiratory tract infection in adults with chronic obstructive pulmonary disease (COPD). The antibody response to outer membrane protein (OMP) CD, a highly conserved surface protein of M. catarrhalis under consideration as a vaccine antigen, was studied in adults with COPD following 40 episodes of infection or colonization. Following infection or colonization, 9 of 40 patients developed new serum immunoglobulin G (IgG) to OMP CD, as measured by enzyme-linked immunosorbent assay. Adsorption assays revealed that a proportion of the serum IgG was directed toward surface-exposed epitopes on OMP CD in six of the nine patients who developed new IgG to OMP CD. Immunoblot assays with fusion peptide constructs indicated that the new antibodies that developed after infection or colonization recognized conformational epitopes, particularly in the carboxy region of the protein. Three of 28 patients developed new mucosal IgA to OMP CD in sputum supernatants. This study establishes that OMP CD is a target of a systemic and mucosal immune response following infection and colonization in some patients with COPD.

* Corresponding author. Mailing address: Buffalo Veterans Affairs Medical Center, Medical Research 151, 3495 Bailey Ave., Buffalo, NY 14215. Phone: (716) 862-7874. Fax: (716) 862-6526. E-mail: murphyt{at}acsu.buffalo.edu.

Editor: D. L. Burns

Infection and Immunity, March 2003, p. 1288-1294, Vol. 71, No. 3
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.3.1288-1294.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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