This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Murphy, T. F.
Right arrow Articles by Sethi, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Murphy, T. F.
Right arrow Articles by Sethi, S.

 Previous Article  |  Next Article 

Infection and Immunity, March 2003, p. 1288-1294, Vol. 71, No. 3
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.3.1288-1294.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Human Immune Response to Outer Membrane Protein CD of Moraxella catarrhalis in Adults with Chronic Obstructive Pulmonary Disease

Timothy F. Murphy,1,2,3* Charmaine Kirkham,1 Dai-Fang Liu,4 and Sanjay Sethi3,5

Divisions of Infectious Diseases,1 Pulmonary and Critical Care Medicine, Department of Medicine,5 Department of Microbiology, University at Buffalo, The State University of New York,2 Veterans Affairs Western New York Healthcare System, Buffalo,3 Wyeth Vaccines Research, West Henrietta, New York4

Received 19 September 2002/ Returned for modification 22 October 2002/ Accepted 29 November 2002

Moraxella catarrhalis is a common cause of lower respiratory tract infection in adults with chronic obstructive pulmonary disease (COPD). The antibody response to outer membrane protein (OMP) CD, a highly conserved surface protein of M. catarrhalis under consideration as a vaccine antigen, was studied in adults with COPD following 40 episodes of infection or colonization. Following infection or colonization, 9 of 40 patients developed new serum immunoglobulin G (IgG) to OMP CD, as measured by enzyme-linked immunosorbent assay. Adsorption assays revealed that a proportion of the serum IgG was directed toward surface-exposed epitopes on OMP CD in six of the nine patients who developed new IgG to OMP CD. Immunoblot assays with fusion peptide constructs indicated that the new antibodies that developed after infection or colonization recognized conformational epitopes, particularly in the carboxy region of the protein. Three of 28 patients developed new mucosal IgA to OMP CD in sputum supernatants. This study establishes that OMP CD is a target of a systemic and mucosal immune response following infection and colonization in some patients with COPD.


* Corresponding author. Mailing address: Buffalo Veterans Affairs Medical Center, Medical Research 151, 3495 Bailey Ave., Buffalo, NY 14215. Phone: (716) 862-7874. Fax: (716) 862-6526. E-mail: murphyt{at}acsu.buffalo.edu.

Editor: D. L. Burns


Infection and Immunity, March 2003, p. 1288-1294, Vol. 71, No. 3
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.3.1288-1294.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.




This article has been cited by other articles:

  • LaFontaine, E. R., Snipes, L. E., Bullard, B., Brauer, A. L., Sethi, S., Murphy, T. F. (2009). Identification of Domains of the Hag/MID Surface Protein Recognized by Systemic and Mucosal Antibodies in Adults with Chronic Obstructive Pulmonary Disease following Clearance of Moraxella catarrhalis. CVI 16: 653-659 [Abstract] [Full Text]  
  • Verhaegh, S. J. C., Streefland, A., Dewnarain, J. K., Farrell, D. J., van Belkum, A., Hays, J. P. (2008). Age-related genotypic and phenotypic differences in Moraxella catarrhalis isolates from children and adults presenting with respiratory disease in 2001-2002. Microbiology 154: 1178-1184 [Abstract] [Full Text]  
  • Ruckdeschel, E. A., Kirkham, C., Lesse, A. J., Hu, Z., Murphy, T. F. (2008). Mining the Moraxella catarrhalis Genome: Identification of Potential Vaccine Antigens Expressed during Human Infection. Infect. Immun. 76: 1599-1607 [Abstract] [Full Text]  
  • Balder, R., Hassel, J., Lipski, S., Lafontaine, E. R. (2007). Moraxella catarrhalis Strain O35E Expresses Two Filamentous Hemagglutinin-Like Proteins That Mediate Adherence to Human Epithelial Cells. Infect. Immun. 75: 2765-2775 [Abstract] [Full Text]  
  • Liu, D.-F., McMichael, J. C., Baker, S. M. (2007). Moraxella catarrhalis Outer Membrane Protein CD Elicits Antibodies That Inhibit CD Binding to Human Mucin and Enhance Pulmonary Clearance of M. catarrhalis in a Mouse Model. Infect. Immun. 75: 2818-2825 [Abstract] [Full Text]  
  • Becker, P. D., Bertot, G. M., Souss, D., Ebensen, T., Guzman, C. A., Grinstein, S. (2007). Intranasal Vaccination with Recombinant Outer Membrane Protein CD and Adamantylamide Dipeptide as the Mucosal Adjuvant Enhances Pulmonary Clearance of Moraxella catarrhalis in an Experimental Murine Model. Infect. Immun. 75: 1778-1784 [Abstract] [Full Text]  
  • Lipski, S. L., Akimana, C., Timpe, J. M., Wooten, R. M., Lafontaine, E. R. (2007). The Moraxella catarrhalis Autotransporter McaP Is a Conserved Surface Protein That Mediates Adherence to Human Epithelial Cells through Its N-Terminal Passenger Domain. Infect. Immun. 75: 314-324 [Abstract] [Full Text]  
  • Murphy, T. F., Brauer, A. L., Aebi, C., Sethi, S. (2005). Antigenic Specificity of the Mucosal Antibody Response to Moraxella catarrhalis in Chronic Obstructive Pulmonary Disease. Infect. Immun. 73: 8161-8166 [Abstract] [Full Text]  
  • Adlowitz, D. G., Sethi, S., Cullen, P., Adler, B., Murphy, T. F. (2005). Human Antibody Response to Outer Membrane Protein G1a, a Lipoprotein of Moraxella catarrhalis. Infect. Immun. 73: 6601-6607 [Abstract] [Full Text]  
  • Murphy, T. F., Brauer, A. L., Aebi, C., Sethi, S. (2005). Identification of Surface Antigens of Moraxella catarrhalis as Targets of Human Serum Antibody Responses in Chronic Obstructive Pulmonary Disease. Infect. Immun. 73: 3471-3478 [Abstract] [Full Text]