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Infection and Immunity, March 2003, p. 1590-1595, Vol. 71, No. 3
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.3.1590-1595.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

DsbA of Pseudomonas aeruginosa Is Essential for Multiple Virulence Factors

Department of Molecular Genetics and Microbiology, University of Florida, Gainesville, Florida 32610

Received 12 July 2002/ Returned for modification 16 October 2002/ Accepted 25 November 2002

DsbA is a periplasmic thiol:disulfide oxidoreductase which contributes to the process of protein folding by catalyzing the formation of disulfide bonds. In this study, we demonstrate that the dsbA gene is required for the expression of the type III secretion system under low-calcium inducing conditions, intracellular survival of P. aeruginosa upon infection of HeLa cells, and twitching motility. The diverse phenotypes of the dsbA mutant are likely due to its defect in the folding of proteins that are involved in various biological processes, such as signal sensing, protein secretion, and defense against host clearing. In light of its effect on various virulence factors, DsbA could be an important target for the control of P. aeruginosa infections.

Editor: V. J. DiRita

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