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Infection and Immunity, March 2003, p. 1599-1603, Vol. 71, No. 3
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.3.1599-1603.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Purification of Fully Activated Clostridium botulinum Serotype B Toxin for Treatment of Patients with Dystonia

Hideyuki Arimitsu,1 Kaoru Inoue,1 Yoshihiko Sakaguchi,1 Jaechul Lee,1 Yukako Fujinaga,1 Toshihiro Watanabe,2 Tohru Ohyama,2 Robert Hirst,3 and Keiji Oguma1*

Department of Bacteriology, Okayama University Graduate School of Medicine and Dentistry, Okayama 700-8558,1 Department of Food Science and Technology, Faculty of Bioindustry, Tokyo University of Agriculture, 196 Yasaka, Abashiri 099-2493, Japan,2 Department of Microbiology and Immunology, James Cook University, Townsville 4811, Australia3

Received 9 September 2002/ Returned for modification 1 November 2002/ Accepted 2 December 2002

Clostridium botulinum serotype B toxins 12S and 16S were separated by using a beta-lactose gel column at pH 6.0; toxin 12S passed through the column, whereas toxin 16S bound to the column and eluted with lactose. The fully activated neurotoxin was obtained by applying the trypsin-treated 16S toxin on the same column at pH 8.0; the neurotoxin passed through the column, whereas remaining nontoxic components bound to the column. The toxicity of this purified fully activated neurotoxin was retained for a long period by addition of albumin in the preparation.

* Corresponding author. Mailing address: Department of Bacteriology, Okayama University Medical School, 2-5-1 Shikata-cho, Okayama 700-8558, Japan. Phone: 81-86-235-7162. Fax: 81-86-235-7162. E-mail: kuma{at}md.okayama-u.ac.jp.

Editor: J. T. Barbieri

Infection and Immunity, March 2003, p. 1599-1603, Vol. 71, No. 3
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.3.1599-1603.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Jin, Y., Takegahara, Y., Sugawara, Y., Matsumura, T., Fujinaga, Y. (2009). Disruption of the epithelial barrier by botulinum haemagglutinin (HA) proteins - differences in cell tropism and the mechanism of action between HA proteins of types A or B, and HA proteins of type C. Microbiology 155: 35-45 [Abstract] [Full Text]  
  • Atassi, M. Z. (2006). On the enhancement of anti-neurotoxin antibody production by subcomponents HA1 and HA3b of Clostridium botulinum type B 16S toxin-haemagglutinin. Microbiology 152: 1891-1895 [Full Text]  
  • Lee, J.-C., Yokota, K., Arimitsu, H., Hwang, H.-J., Sakaguchi, Y., Cui, J., Takeshi, K., Watanabe, T., Ohyama, T., Oguma, K. (2005). Production of anti-neurotoxin antibody is enhanced by two subcomponents, HA1 and HA3b, of Clostridium botulinum type B 16S toxin-haemagglutinin. Microbiology 151: 3739-3747 [Abstract] [Full Text]  
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