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Infection and Immunity, April 2003, p. 1656-1661, Vol. 71, No. 4
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.4.1656-1661.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Virulence, Immunogenicity, and Protective Efficacy of Two Recombinant Mycobacterium bovis Bacillus Calmette-Guérin Strains Expressing the Antigen ESAT-6 from Mycobacterium tuberculosis

Lang Bao,* Wei Chen, Huidong Zhang, and Xiaoying Wang

Research Unit of Infection and Immunity, West China Medical Center, Sichuan University, Chengdu, Sichuan 610041, People's Republic of China

Received 16 September 2002/ Returned for modification 31 October 2002/ Accepted 3 January 2003

We constructed two recombinant Mycobacterium bovis BCG (rBCG) strains expressing ESAT-6 of Mycobacterium tuberculosis, named rBCG-1 and rBCG-2. rBCG-1 contained the ESAT-6 gene linked to BCG hsp60 and expressed a fusion protein, while rBCG-2, with a secretory sequence, could secret ESAT-6 into the culture medium. There was no evidence for increased virulence of the two rBCG strains when we made a comparison between them and BCG with regard to organ bacterial loads, lung histology, and survival time. rBCG-1 induced significantly higher specific antibody titers and stronger cellular immune response than BCG, whereas rBCG-2 had immunogenicity similar to that of the parental BCG strain. Both rBCG-1 and rBCG-2 conferred marked protection against M. tuberculosis infection, yet in terms of protective efficacy, they showed no significant improvements upon conventional BCG vaccine.

* Corresponding author. Mailing address: No. 17, 3rd Section, Ren Min Nan Rd., Chengdu, Sichuan Province 610041, People's Republic of China. Phone: 86 28 85 40 79 19. Fax: 86 28 85 50 10 07. E-mail: baolang{at}wcums.edu.cn.

Editor: J. D. Clements

Infection and Immunity, April 2003, p. 1656-1661, Vol. 71, No. 4
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.4.1656-1661.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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