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Infection and Immunity, April 2003, p. 1774-1783, Vol. 71, No. 4
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.4.1774-1783.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Afa/Dr Diffusely Adhering Escherichia coli Infection in T84 Cell Monolayers Induces Increased Neutrophil Transepithelial Migration, Which in Turn Promotes Cytokine-Dependent Upregulation of Decay-Accelerating Factor (CD55), the Receptor for Afa/Dr Adhesins

Fréderic Bétis,1 Patrick Brest,1 Véronique Hofman,2 Julie Guignot,3 Imad Kansau,3 Bernard Rossi,1 Alain Servin,3 and Paul Hofman1,2*

Unité INSERM 364,1 Laboratoire d'Anatomie Pathologique, Faculté de Médecine, 06107 Nice Cédex 02,2 Unité INSERM 510, Faculté de Pharmacie Paris XI, 92296 ChÂtenay-Malabry, France3

Received 21 August 2002/ Returned for modification 23 October 2002/ Accepted 3 January 2003

Ulcerative colitis and Crohn's disease are inflammatory bowel diseases thought to involve strains of Escherichia coli. We report here that two wild-type Afa/Dr diffusely adhering E. coli (DAEC) strains, C1845 and IH11128, which harbor the fimbrial F1845 adhesin and the Dr hemagglutinin, respectively, and the E. coli laboratory strain HB101, transformed with the pSSS1 plasmid to produce Afa/Dr F1845 adhesin, all induced interleukin-8 (IL-8) production and transepithelial migration of polymorphonuclear leukocytes (PMNL) in polarized monolayers of the human intestinal cell line T84 grown on semipermeable filters. We observed that after PMNL migration, expression of decay-accelerating factor (DAF, or CD55), the brush border-associated receptor for Afa/Dr adhesins, was strongly enhanced, increasing the adhesion of Afa/Dr DAEC bacteria. When examining the mechanism by which DAF expression was enhanced, we observed that the PMNL transepithelial migration induced epithelial synthesis of tumor necrosis factor alpha and IL-1ß, which in turn promoted the upregulation of DAF.

* Corresponding author. Mailing address: INSERM Unité 364, IFR 50, Faculté de Médecine, avenue de Valombrose, 06107 Nice, Cédex 02, France. Phone: 33 4 93 37 77 07. Fax: 33 4 93 81 94 56. E-mail: hofman{at}unice.fr.

Editor: V. J. DiRita

Infection and Immunity, April 2003, p. 1774-1783, Vol. 71, No. 4
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.4.1774-1783.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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