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Infection and Immunity, April 2003, p. 1820-1827, Vol. 71, No. 4
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.4.1820-1827.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Protective Efficacy of Anti-Helicobacter pylori Immunity following Systemic Immunization of Neonatal Mice

Julia C. Eisenberg,1 Steven J. Czinn,2 Christine A. Garhart,1 Raymond W. Redline,1 Wolf C. Bartholomae,1 Judith M. Gottwein,1 John G. Nedrud,1 Steven E. Emancipator,1 Bernhard B. Boehm,3 Paul V. Lehmann,1 and Thomas G. Blanchard2*

Departments of Pathology,1 Pediatrics, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106,2 Section of Endocrinology, University Hospitals of Ulm, 89081 Ulm, Germany3

Received 15 October 2002/ Returned for modification 26 November 2002/ Accepted 9 January 2003

Helicobacter pylori infection of the gastric mucosa is a significant cause of morbidity and mortality because of its etiologic role in symptomatic gastritis, peptic ulcer disease, and gastric adenocarcinoma. Infection occurs in young children; therefore, a prophylactic vaccine would have to be administered within the first year of life, a period thought to be immunologically privileged. We investigated vaccine formulations administered by different routes to confer protective anti-H. pylori immunity in neonatal mice. Neonatal mice immunized with a single dose of vaccine in complete Freund's adjuvant (CFA) generated antigen-specific gamma interferon-, interleukin-2 (IL-2)-, IL-4-, and IL-5-secreting T cells in numbers similar to those in immunized adult mice, while vaccine administered to neonates in incomplete Freund's adjuvant (IFA) induced such cells in reduced numbers compared to those in adult mice. Both IFA and CFA, however, provided partial protection from a challenge with infectious H. pylori when the vaccine was administered subcutaneously. Neonatal immunized mice also had reduced bacterial loads when immunized intraperitoneally with CFA. In all cases, protection was equivalent to that achieved when adult counterparts were immunized. These studies suggest that an efficacious vaccine might be successfully administered to very young children to prevent perinatal infection of H. pylori.

* Corresponding author. Mailing address: Department of Pediatrics, Rm. 737, Rainbow Babies & Children's Hospital, 11100 Euclid Ave., Cleveland, OH 44106. Phone: (216) 844-7363. Fax: (216) 844-7642. E-mail: tgb4{at}po.cwru.edu.

Editor: J. D. Clements

Infection and Immunity, April 2003, p. 1820-1827, Vol. 71, No. 4
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.4.1820-1827.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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