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Infection and Immunity, April 2003, p. 1843-1848, Vol. 71, No. 4
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.4.1843-1848.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Inactivation of the Moraxella catarrhalis 7169 Ferric Uptake Regulator Increases Susceptibility to the Bactericidal Activity of Normal Human Sera

Kristin Furano1,2 and Anthony A. Campagnari1,2,3*

Department of Microbiology,1 Witebsky Center for Microbial Pathogenesis and Immunology,2 Division of Infectious Diseases, Department of Medicine, State University of New York at Buffalo, Buffalo, New York 142143

Received 5 November 2002/ Returned for modification 10 December 2002/ Accepted 3 January 2003

Moraxella catarrhalis is a strict human pathogen and a significant cause of respiratory disease and otitis media. In direct response to these infections, research efforts have focused primarily on the identification of potential vaccine targets. The general biology of M. catarrhalis, however, including the mechanisms utilized to survive in the human host, remains poorly understood. Previous work has demonstrated that M. catarrhalis expresses iron-repressible proteins, suggesting the presence of iron acquisition systems under the control of a ferric uptake regulator (Fur). In this study M. catarrhalis fur has been cloned and sequenced from strain 7169. A deletion-insertion mutation of 7169 fur resulted in upregulation of iron-repressible outer membrane proteins in the absence and presence of iron. This mutant strain, 7169fur1, was significantly more sensitive to the bactericidal activity of normal human serum than the resistant wild-type strain. These data suggest that constitutive expression of iron-regulated proteins may provide multiple targets for human antibodies. In addition, the 7169 fur mutant provides an important tool for further investigation of the iron acquisition mechanisms utilized by M. catarrhalis.

* Corresponding author. Mailing address: 140 Biomedical Research Building, State University of New York at Buffalo, 3435 Main St., Buffalo, NY 14214. Phone: (716) 829-2673. Fax: (716) 829-3889. E-mail: AAC{at}acsu.buffalo.edu.

Editor: D. L. Burns

Infection and Immunity, April 2003, p. 1843-1848, Vol. 71, No. 4
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.4.1843-1848.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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