This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Vordermeier, M.
Right arrow Articles by Hewinson, R. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Vordermeier, M.
Right arrow Articles by Hewinson, R. G.

 Previous Article  |  Next Article 

Infection and Immunity, April 2003, p. 1980-1987, Vol. 71, No. 4
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.4.1980-1987.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Recognition of Mycobacterial Epitopes by T Cells across Mammalian Species and Use of a Program That Predicts Human HLA-DR Binding Peptides To Predict Bovine Epitopes

Martin Vordermeier,* Adam O. Whelan, and R. Glyn Hewinson

TB Research Group, Veterinary Laboratories Agency-Weybridge, New Haw, Addlestone, Surrey KT15 3NB, United Kingdom

Received 17 September 2002/ Returned for modification 7 November 2002/ Accepted 9 January 2003

Bioinformatics tools have the potential to accelerate research into the design of vaccines and diagnostic tests by exploiting genome sequences. The aim of this study was to assess whether in silico analysis could be combined with in vitro screening methods to rapidly identify peptides that are immunogenic during Mycobacterium bovis infection of cattle. In the first instance the M. bovis-derived protein ESAT-6 was used as a model antigen to describe peptides containing T-cell epitopes that were frequently recognized across mammalian species, including natural hosts for tuberculosis (humans and cattle) and small-animal models of tuberculosis (mice and guinea pigs). Having demonstrated that some peptides could be recognized by T cells from a number of M. bovis-infected hosts, we tested whether a virtual-matrix-based human prediction program (ProPred) could identify peptides that were recognized by T cells from M. bovis-infected cattle. In this study, 73% of the experimentally defined peptides from 10 M. bovis antigens that were recognized by bovine T cells contained motifs predicted by ProPred. Finally, in validating this observation, we showed that three of five peptides from the mycobacterial antigen Rv3019c that were predicted to contain HLA-DR-restricted epitopes were recognized by T cells from M. bovis-infected cattle. The results obtained in this study support the approach of using bioinformatics to increase the efficiency of epitope screening and selection.

* Corresponding author. Mailing address: TB Research Group, Veterinary Laboratories Agency-Weybridge, New Haw, Addlestone, Surrey KT15 3NB, United Kingdom. Phone: 44 1932 357 884. Fax: 44 1932 357 684. E-mail: mvordermeier.vla{at}gtnet.gov.uk.

Editor: S. H. E Kaufmann

Infection and Immunity, April 2003, p. 1980-1987, Vol. 71, No. 4
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.4.1980-1987.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Sidders, B., Pirson, C., Hogarth, P. J., Hewinson, R. G., Stoker, N. G., Vordermeier, H. M., Ewer, K. (2008). Screening of Highly Expressed Mycobacterial Genes Identifies Rv3615c as a Useful Differential Diagnostic Antigen for the Mycobacterium tuberculosis Complex. Infect. Immun. 76: 3932-3939 [Abstract] [Full Text]  
  • Vordermeier, H. M., Brown, J., Cockle, P. J., Franken, W. P. J., Arend, S. M., Ottenhoff, T. H. M., Jahans, K., Hewinson, R. G. (2007). Assessment of Cross-Reactivity between Mycobacterium bovis and M. kansasii ESAT-6 and CFP-10 at the T-Cell Epitope Level. CVI 14: 1203-1209 [Abstract] [Full Text]  
  • Cockle, P. J., Gordon, S. V., Hewinson, R. G., Vordermeier, H. M. (2006). Field Evaluation of a Novel Differential Diagnostic Reagent for Detection of Mycobacterium bovis in Cattle. CVI 13: 1119-1124 [Abstract] [Full Text]  
  • Mustafa, A. S., Skeiky, Y. A., Al-Attiyah, R., Alderson, M. R., Hewinson, R. G., Vordermeier, H. M. (2006). Immunogenicity of Mycobacterium tuberculosis Antigens in Mycobacterium bovis BCG-Vaccinated and M. bovis-Infected Cattle.. Infect. Immun. 74: 4566-4572 [Abstract] [Full Text]  
  • Ewer, K., Cockle, P., Gordon, S., Mansoor, H., Govaerts, M., Walravens, K., Marche, S., Hewinson, G., Vordermeier, M. (2006). Antigen Mining with Iterative Genome Screens Identifies Novel Diagnostics for the Mycobacterium tuberculosis Complex. CVI 13: 90-97 [Abstract] [Full Text]  
  • Collins, N. E., Liebenberg, J., de Villiers, E. P., Brayton, K. A., Louw, E., Pretorius, A., Faber, F. E., van Heerden, H., Josemans, A., van Kleef, M., Steyn, H. C., van Strijp, M. F., Zweygarth, E., Jongejan, F., Maillard, J. C., Berthier, D., Botha, M., Joubert, F., Corton, C. H., Thomson, N. R., Allsopp, M. T., Allsopp, B. A. (2005). The genome of the heartwater agent Ehrlichia ruminantium contains multiple tandem repeats of actively variable copy number. Proc. Natl. Acad. Sci. USA 102: 838-843 [Abstract] [Full Text]  
  • Vordermeier, H. M., Simsova, M., Wilkinson, K. A., Wilkinson, R. J., Hewinson, R. G., Sebo, P., Leclerc, C. (2004). Recognition of Mycobacterial Antigens Delivered by Genetically Detoxified Bordetella pertussis Adenylate Cyclase by T Cells from Cattle with Bovine Tuberculosis. Infect. Immun. 72: 6255-6261 [Abstract] [Full Text]  
  • Demangel, C., Brodin, P., Cockle, P. J., Brosch, R., Majlessi, L., Leclerc, C., Cole, S. T. (2004). Cell Envelope Protein PPE68 Contributes to Mycobacterium tuberculosis RD1 Immunogenicity Independently of a 10-Kilodalton Culture Filtrate Protein and ESAT-6. Infect. Immun. 72: 2170-2176 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»