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Infection and Immunity, April 2003, p. 2130-2141, Vol. 71, No. 4
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.4.2130-2141.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

CesD2 of Enteropathogenic Escherichia coli Is a Second Chaperone for the Type III Secretion Translocator Protein EspD

Bianca C. Neves,1 Rosanna Mundy,1 Liljana Petrovska,1 Gordon Dougan,1 Stuart Knutton,2 and Gad Frankel1*

Centre for Molecular Microbiology and Infection, Department of Biological Sciences, Imperial College London, London SW7 2AZ,1 Institute of Child Health, University of Birmingham, Birmingham B4 6NH, United Kingdom2

Received 23 September 2002/ Returned for modification 15 November 2002/ Accepted 20 December 2002

Enteropathogenic Escherichia coli (EPEC) and enterohemorrhagic E. coli are extracellular pathogens that employ a type III secretion system to export translocator and effector proteins, proteins which facilitates colonization of the mucosal surface of the intestine via formation of attaching and effacing (A/E) lesions. The genes encoding the proteins for A/E lesion formation are located on a pathogenicity island, termed the locus of enterocyte effacement (LEE), which contains eae encoding intimin as well as the type III secretion system and effector genes. Many type III secreted proteins are stabilized and maintained in a secretion-competent conformation in the bacterial cytosol by specific chaperone proteins. Three type III chaperones have been described thus far within the EPEC LEE region: CesD, for the translocator proteins EspB and EspD; CesT, for the effector proteins Tir and Map; and CesF, for EspF. In this study we report the characterization of CesD2 (previously Orf27), a second LEE-encoded chaperone for EspD. We show specific CesD2-EspD protein interaction which appears to be necessary for proper EspD secretion in vitro and pathogenesis in vivo as demonstrated in the A/E-lesion-forming mouse pathogen Citrobacter rodentium.

* Corresponding author. Mailing address: Centre for Molecular Microbiology and Infection, Flowers Building, Department of Biological Sciences, Imperial College, London SW7 2AZ, United Kingdom. Phone: 44-(0)20-7594-5253. Fax: 44-(0)20-7594-3069. E-mail: g.frankel{at}ic.ac.uk.

Editor: J. T. Barbieri

Infection and Immunity, April 2003, p. 2130-2141, Vol. 71, No. 4
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.4.2130-2141.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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