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Mucosal Immunization with a Genetically Engineered Pertussis Toxin S1 Fragment-Cholera Toxin Subunit B Chimeric Protein

Department of Applied Oral Sciences, Faculty of Dentistry,¹ Department of Microbiology & Immunology, Dalhousie University,² Department of Pediatrics, IWK Health Centre, Halifax, Nova Scotia, Canada B3H 3J5³

Received 6 November 2002/ Returned for modification 22 November 2002/ Accepted 13 December 2002

A chimeric protein consisting of a divalent pertussis toxin (PT) S1 fragment linked to the cholera toxin (Ctx) A₂B fragment was constructed. The chimera induced a mucosal immunoglobulin A (IgA) and a serum IgG immune response to PT and CtxB in BALB/c mice following intranasal immunization. The immune sera neutralized PT in vitro. In the mouse model of Bordetella pertussis respiratory infection, the chimera-immunized animals showed a significant reduction in bacterial lung counts (P = 0.01) from that of the sham control group. Thus, a divalent S1 fragment CtxA2B chimera is an immunogenic antigen and can elicit a protective immunity.

Editor: J. T. Barbieri

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