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Infection and Immunity, April 2003, p. 2272-2275, Vol. 71, No. 4
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.4.2272-2275.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Mucosal Immunization with a Genetically Engineered Pertussis Toxin S1 Fragment-Cholera Toxin Subunit B Chimeric Protein

Song F. Lee,1,2* Scott A. Halperin,2,3 Danny F. Salloum,2 Ann MacMillan,3 and Annette Morris3

Department of Applied Oral Sciences, Faculty of Dentistry,1 Department of Microbiology & Immunology, Dalhousie University,2 Department of Pediatrics, IWK Health Centre, Halifax, Nova Scotia, Canada B3H 3J53

Received 6 November 2002/ Returned for modification 22 November 2002/ Accepted 13 December 2002

A chimeric protein consisting of a divalent pertussis toxin (PT) S1 fragment linked to the cholera toxin (Ctx) A2B fragment was constructed. The chimera induced a mucosal immunoglobulin A (IgA) and a serum IgG immune response to PT and CtxB in BALB/c mice following intranasal immunization. The immune sera neutralized PT in vitro. In the mouse model of Bordetella pertussis respiratory infection, the chimera-immunized animals showed a significant reduction in bacterial lung counts (P = 0.01) from that of the sham control group. Thus, a divalent S1 fragment CtxA2B chimera is an immunogenic antigen and can elicit a protective immunity.

* Corresponding author. Mailing address: Department of Applied Oral Sciences, Faculty of Dentistry, Dalhousie University, Halifax, Nova Scotia, Canada B3H 3J5. Phone: (902) 494-8799. Fax: (902) 494-6621. E-mail: song.lee{at}dal.ca.

Editor: J. T. Barbieri

Infection and Immunity, April 2003, p. 2272-2275, Vol. 71, No. 4
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.4.2272-2275.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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