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Infection and Immunity, May 2003, p. 2326-2330, Vol. 71, No. 5
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.5.2326-2330.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Brucella abortus RB51 Induces Protection in Mice Orally Infected with the Virulent Strain B. abortus 2308

Paolo Pasquali,* Adone Rosanna, Claudia Pistoia, Paola Petrucci, and Franco Ciuchini

Laboratory of Veterinary Medicine, Istituto Superiore di Sanità, Rome, Italy

Received 24 June 2002/ Returned for modification 3 December 2002/ Accepted 15 January 2003

Brucellae are gram-negative, facultative intracellular bacteria which are one of the most common causes of abortion in animals. In addition, they are the source of a severe zoonosis. In this trial, we evaluated the effect of oral inoculation of Brucella abortus RB51 in mice against a challenge infection with B. abortus 2308. First, we showed that a gastric acid neutralization prior to the oral inoculation contributed to a more homogeneous and consistent infection with both vaccine strain B. abortus RB51 and virulent strain B. abortus 2308. Successively, we assessed the clearance and the immune response following an oral infection with B. abortus RB51. Oral inoculation gave a mild infection which was cleared 42 days after infection, and it induced a delayed humoral and cell-mediated immune response. Finally, we immunized mice by oral inoculation with B. abortus RB51, and we challenged them with the virulent strain B. abortus 2308 by an oral or intraperitoneal route 42 days after vaccination. Oral inoculation of B. abortus RB51 was able to give protection to mice infected with the virulent strain B. abortus 2308 by the oral route but not to mice infected intraperitoneally. Our results indicate that oral inoculation of mice with B. abortus RB51 is able to give a protective immunity against an oral infection with virulent strains, and this protection seems to rely on an immune response at the mucosal level.

* Corresponding author. Mailing address: Laboratory of Veterinary Medicine, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy. Phone: 39 06 49902728. Fax: 39 06 49387077. E-mail: pasquali{at}iss.it.

Editor: V. J. DiRita

Infection and Immunity, May 2003, p. 2326-2330, Vol. 71, No. 5
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.5.2326-2330.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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