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Infection and Immunity, May 2003, p. 2684-2692, Vol. 71, No. 5
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.5.2684-2692.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Persistence of Mucosal Mast Cells and Eosinophils in Shigella-Infected Children

Rubhana Raqib,1* Pricila Khan Moly,1 Protim Sarker,1 Firdausi Qadri,1 Nurul Haque Alam,1 Minnie Mathan,1 and Jan Andersson2

International Centre for Diarrhoeal Diseases Research, Bangladesh (ICDDR, B), Dhaka, Bangladesh,1 Department of Medicine, Center for Infectious Diseases, Huddinge University Hospital, Karolinska Institutet, Stockholm, Sweden2

Received 4 October 2002/ Returned for modification 30 December 2002/ Accepted 6 February 2003

Cells of the innate immune system and their mediators were studied at the single-cell level in the rectums of pediatric and adult patients with Shigella infection to better understand why children are at higher risk for severe infection. Adult patients had increased infiltration of mucosal mast cells (MMC) at the acute stage (3 to 5 days after the onset of diarrhea) and eosinophils in early convalescence (14 to 16 days after onset). Increased expression of stem cell factor and prostaglandin H synthase-1 (PGHS-1) was associated with increased tryptase-Ki67-double-positive MMC in the acute stage and increased apoptosis of MMC, which led to a rapid decline in early convalescence. The eosinophils demonstrated increased expression of major basic protein (MBP), eotaxin, and CCR3, as well as increased necrotic death. The neutrophils showed enhanced {alpha}-defensin and lactoferrin expression in the acute phase. In contrast to adults, the pediatric patients demonstrated delayed accumulation of mast cells and eosinophils, while {alpha}-defensin expression persisted during convalescence. In contrast, neutrophil counts and lactoferrin expression were reduced in children compared to adults. The results suggest that children with shigellosis have a persistent activation of the innate immune response in the convalescent phase, indicating delayed elimination of Shigella antigens compared to adults.

* Corresponding author. Mailing address: Immunology Laboratory, Laboratory Sciences Division, ICDDR, B, Mohakhali, Dhaka-1212, Bangladesh. Phone: 880-2-8811751-60, ext. 2404. Fax: 880-2-8823116/8812529/8825060. E-mail: rubhana{at}icddrb.org.

Editor: F. C. Fang

Infection and Immunity, May 2003, p. 2684-2692, Vol. 71, No. 5
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.5.2684-2692.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Qadri, F, Bhuiyan, T R, Dutta, K K, Raqib, R, Alam, M S, Alam, N H, Svennerholm, A-M, Mathan, M M (2004). Acute dehydrating disease caused by Vibrio cholerae serogroups O1 and O139 induce increases in innate cells and inflammatory mediators at the mucosal surface of the gut. Gut 53: 62-69 [Abstract] [Full Text]  


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