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Infection and Immunity, May 2003, p. 2876-2880, Vol. 71, No. 5
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.5.2876-2880.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Limited Role of Lipopolysaccharide Lewis Antigens in Adherence of Helicobacter pylori to the Human Gastric Epithelium

Jafar Mahdavi,1 Thomas Borén,1 Christina Vandenbroucke-Grauls,2 and Ben J. Appelmelk2*

Department of Odontology/Oral Microbiology, Umeå University, 901 87 Umeå, Sweden,1 Department of Medical Microbiology, Vrije Universiteit Medical Center, 1081 BT Amsterdam, The Netherlands2

Received 3 October 2002/ Returned for modification 18 November 2002/ Accepted 17 January 2003

In vitro and in vivo studies from various groups have suggested that Helicobacter pylori lipopolysaccharide (LPS) Lewis x (Lex) antigens mediate bacterial adhesion. We have now reevaluated this hypothesis by studying the adherence in situ of H. pylori strain 11637 and its corresponding Lex-negative rfbM mutant to human gastric mucosa from patients (n = 22) with various gastric pathologies. Significant binding of the parent strain was observed in only 8 out of 22 sections; in four out of eight patients, the Lex-negative mutant bound less well. One of these four patients displayed no gastric abnormalities, and the other three showed dysplasia, metaplasia, and adenocarcinoma, respectively; hence, we are unable to define the circumstances under which LPS-mediated adhesion takes place. We conclude that H. pylori LPS plays a distinct but minor role in adhesion.

* Corresponding author. Mailing address: Department of Medical Microbiology, VUMC Vrije Universiteit Medical Center, van der Boechorststraat 7, 1081 BT Amsterdam, The Netherlands. Phone: 31 20 4448297. Fax: 31 20 4448318. E-mail: BJ.Appelmelk.mm{at}med.vu.nl.

Editor: J. D. Clements

Infection and Immunity, May 2003, p. 2876-2880, Vol. 71, No. 5
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.5.2876-2880.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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