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Infection and Immunity, May 2003, p. 2885-2891, Vol. 71, No. 5
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.5.2885-2891.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Coordinate Regulation of Salmonella enterica Serovar Typhimurium Invasion of Epithelial Cells by the Arp2/3 Complex and Rho GTPases

Alison K. Criss{dagger} and James E. Casanova*

Department of Cell Biology, University of Virginia Health Sciences Center, Charlottesville, Virginia 22908-0732

Received 18 September 2002/ Returned for modification 8 January 2003/ Accepted 29 January 2003

Salmonella enterica serovar Typhimurium can infect epithelial cells via the basolateral surface after breaching the intestinal epithelium, yet little is known about this process. Here, we show that actin polymerization driven by the Arp2/3 complex is critical to both basolateral and apical bacterial invasion of polarized MDCK cells. While there is also a dependence upon toxin B-sensitive Rho GTPases, none of the four GTPases known to be activated by S. enterica serovar Typhimurium SopE are individually required for basolateral internalization. These results underscore that the specific factors required for Salmonella invasion differ between membrane domains of polarized epithelia.

* Corresponding author. Mailing address: Department of Cell Biology, University of Virginia Health Sciences Center, Box 800732, 1300 Jefferson Park Ave., Charlottesville, VA 22908-0732. Phone: (434) 243-4821. Fax: (434) 982-3912. E-mail: jec9e{at}virginia.edu.

Editor: A. D. O'Brien

{dagger} Present address: Department of Microbiology-Immunology, Northwestern University Feinberg School of Medicine, Chicago, IL 60611.

Infection and Immunity, May 2003, p. 2885-2891, Vol. 71, No. 5
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.5.2885-2891.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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