This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hübner, A.
Right arrow Articles by Norgard, M. V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hübner, A.
Right arrow Articles by Norgard, M. V.

 Previous Article  |  Next Article 

Infection and Immunity, May 2003, p. 2892-2896, Vol. 71, No. 5
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.5.2892-2896.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Expression of a luxS Gene Is Not Required for Borrelia burgdorferi Infection of Mice via Needle Inoculation

Anette Hübner, Andrew T. Revel, Dena M. Nolen, Kayla E. Hagman, and Michael V. Norgard*

Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, Texas 75390

Received 12 December 2002/ Returned for modification 17 January 2003/ Accepted 6 February 2003

The luxS gene product is an integral component of LuxS/autoinducer-2 (AI-2) quorum-sensing systems in bacteria. A putative luxS gene was expressed at comparable levels by Borrelia burgdorferi strain 297 cultivated either in vitro or in dialysis membrane chambers implanted in rat peritoneal cavities. Although the borrelial luxS gene functionally complemented a LuxS deficiency in Escherichia coli DH5{alpha}, AI-2-like activity could not be detected within B. burgdorferi culture supernatants or concentrated cell lysates. Finally, a luxS-deficient mutant of B. burgdorferi was infectious at wild-type levels when it was intradermally needle inoculated into mice, indicating that expression of luxS probably is not required for infectivity but, at the very least, is not essential for mammalian host adaptation. Our findings also challenge the notion that a LuxS/AI-2 quorum-sensing system is operative in B. burgdorferi.

* Corresponding author. Mailing address: Department of Microbiology, U.T. Southwestern Medical Center, 6000 Harry Hines Blvd., Dallas, TX 75390-9048. Phone: (214) 648-5900. Fax: (214) 648-5905. E-mail: michael.norgard{at}utsouthwestern.edu.

Editor: J. T. Barbieri

Infection and Immunity, May 2003, p. 2892-2896, Vol. 71, No. 5
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.5.2892-2896.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Liveris, D., Mulay, V., Sandigursky, S., Schwartz, I. (2008). Borrelia burgdorferi vlsE Antigenic Variation Is Not Mediated by RecA. Infect. Immun. 76: 4009-4018 [Abstract] [Full Text]  
  • Stewart, P. E., Bestor, A., Cullen, J. N., Rosa, P. A. (2008). A Tightly Regulated Surface Protein of Borrelia burgdorferi Is Not Essential to the Mouse-Tick Infectious Cycle. Infect. Immun. 76: 1970-1978 [Abstract] [Full Text]  
  • Riley, S. P., Bykowski, T., Babb, K., von Lackum, K., Stevenson, B. (2007). Genetic and physiological characterization of the Borrelia burgdorferi ORF BB0374-pfs-metK-luxS operon. Microbiology 153: 2304-2311 [Abstract] [Full Text]  
  • von Lackum, K., Ollison, K. M., Bykowski, T., Nowalk, A. J., Hughes, J. L., Carroll, J. A., Zuckert, W. R., Stevenson, B. (2007). Regulated synthesis of the Borrelia burgdorferi inner-membrane lipoprotein IpLA7 (P22, P22-A) during the Lyme disease spirochaete's mammal-tick infectious cycle. Microbiology 153: 1361-1371 [Abstract] [Full Text]  
  • Li, X., Liu, X., Beck, D. S., Kantor, F. S., Fikrig, E. (2006). Borrelia burgdorferi Lacking BBK32, a Fibronectin-Binding Protein, Retains Full Pathogenicity.. Infect. Immun. 74: 3305-3313 [Abstract] [Full Text]  
  • Parveen, N., Cornell, K. A., Bono, J. L., Chamberland, C., Rosa, P., Leong, J. M. (2006). Bgp, a Secreted Glycosaminoglycan-Binding Protein of Borrelia burgdorferi Strain N40, Displays Nucleosidase Activity and Is Not Essential for Infection of Immunodeficient Mice.. Infect. Immun. 74: 3016-3020 [Abstract] [Full Text]  
  • Ojaimi, C., Mulay, V., Liveris, D., Iyer, R., Schwartz, I. (2005). Comparative Transcriptional Profiling of Borrelia burgdorferi Clinical Isolates Differing in Capacities for Hematogenous Dissemination. Infect. Immun. 73: 6791-6802 [Abstract] [Full Text]  
  • Babb, K., von Lackum, K., Wattier, R. L., Riley, S. P., Stevenson, B. (2005). Synthesis of Autoinducer 2 by the Lyme Disease Spirochete, Borrelia burgdorferi. J. Bacteriol. 187: 3079-3087 [Abstract] [Full Text]  
  • Cluss, R. G., Silverman, D. A., Stafford, T. R. (2004). Extracellular Secretion of the Borrelia burgdorferi Oms28 Porin and Bgp, a Glycosaminoglycan Binding Protein. Infect. Immun. 72: 6279-6286 [Abstract] [Full Text]  
  • Blevins, J. S., Revel, A. T., Caimano, M. J., Yang, X. F., Richardson, J. A., Hagman, K. E., Norgard, M. V. (2004). The luxS Gene Is Not Required for Borrelia burgdorferi Tick Colonization, Transmission to a Mammalian Host, or Induction of Disease. Infect. Immun. 72: 4864-4867 [Abstract] [Full Text]  
  • Liveris, D., Mulay, V., Schwartz, I. (2004). Functional Properties of Borrelia burgdorferi recA. J. Bacteriol. 186: 2275-2280 [Abstract] [Full Text]  
  • Anderton, J. M., Tokarz, R., Thill, C. D., Kuhlow, C. J., Brooks, C. S., Akins, D. R., Katona, L. I., Benach, J. L. (2004). Whole-Genome DNA Array Analysis of the Response of Borrelia burgdorferi to a Bactericidal Monoclonal Antibody. Infect. Immun. 72: 2035-2044 [Abstract] [Full Text]  
  • Yang, X. F., Pal, U., Alani, S. M., Fikrig, E., Norgard, M. V. (2004). Essential Role for OspA/B in the Life Cycle of the Lyme Disease Spirochete. JEM 199: 641-648 [Abstract] [Full Text]  
  • Frank, K. L., Bundle, S. F., Kresge, M. E., Eggers, C. H., Samuels, D. S. (2003). aadA Confers Streptomycin Resistance in Borrelia burgdorferi. J. Bacteriol. 185: 6723-6727 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»