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Infection and Immunity, May 2003, p. 2911-2915, Vol. 71, No. 5
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.5.2911-2915.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

fhuA of Actinobacillus pleuropneumoniae Encodes a Ferrichrome Receptor but Is Not Regulated by Iron

Leonie G. Mikael,1 Ramakrishnan Srikumar,2 James W. Coulton,2,3 and Mario Jacques1,3*

Groupe de Recherche sur les Maladies Infectieuses du Porc, Département de pathologie et microbiologie, Faculté de médecine vétérinaire, Université de Montréal, and,1 Canadian Research Network on Bacterial Pathogens of Swine, Saint-Hyacinthe, Québec, Canada J2S 7C6, and,3 Department of Microbiology and Immunology, McGill University, Montréal, Québec, Canada H3A 2B42

Received 7 October 2002/ Returned for modification 20 December 2002/ Accepted 6 February 2003

The swine pathogen Actinobacillus pleuropneumoniae possesses a 75-kDa outer membrane protein (OMP), FhuA, the receptor for ferrichrome, a hydroxamate-type siderophore. Polyclonal serum to FhuA reacted with OMP preparations from 12 serotypes of A. pleuropneumoniae under conditions of iron repletion and restriction. Reverse transcription-PCR confirmed that A. pleuropneumoniae fhuA expression is not upregulated in response to low iron levels. An A. pleuropneumoniae fhuA deletion mutant was generated and showed abolishment of ferrichrome uptake.

* Corresponding author. Mailing address: Groupe de Recherche sur les Maladies Infectieuses du Porc, Département de pathologie et microbiologie, Faculté de médecine vétérinaire, Université de Montréal, Saint-Hyacinthe, Québec, Canada J2S 7C6. Phone: (450) 773-8521, ext. 8348. Fax: (450) 778-8108. E-mail: mario.jacques{at}umontreal.ca.

Editor: F. C. Fang

Infection and Immunity, May 2003, p. 2911-2915, Vol. 71, No. 5
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.5.2911-2915.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Nielsen, K. K., Boye, M. (2005). Real-Time Quantitative Reverse Transcription-PCR Analysis of Expression Stability of Actinobacillus pleuropneumoniae Housekeeping Genes during In Vitro Growth under Iron-Depleted Conditions. Appl. Environ. Microbiol. 71: 2949-2954 [Abstract] [Full Text]  
  • Srikumar, R., Mikael, L. G., Pawelek, P. D., Khamessan, A., Gibbs, B. F., Jacques, M., Coulton, J. W. (2004). Molecular cloning of haemoglobin-binding protein HgbA in the outer membrane of Actinobacillus pleuropneumoniae. Microbiology 150: 1723-1734 [Abstract] [Full Text]  


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