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Infection and Immunity, May 2003, p. 2916-2919, Vol. 71, No. 5
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.5.2916-2919.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Characterization of Staphylococcus aureus Enterotoxin L

Paul M. Orwin,1,{dagger} J. Ross Fitzgerald,2,{ddagger} Donald Y. M. Leung,3 Juan A. Gutierrez,3 Gregory A. Bohach,4 and Patrick M. Schlievert1*

Department of Microbiology, University of Minnesota Medical School, Minneapolis, Minnesota 55455,1 Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840,2 Department of Pediatrics, National Jewish Medical and Research Center, Denver, Colorado 80206,3 Department of Microbiology, Molecular Biology, and Biochemistry, University of Idaho, Moscow, Idaho 838444

Received 26 November 2002/ Returned for modification 24 January 2003/ Accepted 12 February 2003

Staphylococcus aureus causes a wide variety of diseases. Major virulence factors of this organism include enterotoxins (SEs) that cause both food poisoning and toxic shock syndrome. Recently, a novel SE, tentatively designated SEL, was identified in a pathogenicity island from a bovine mastitis isolate. The toxin had a molecular weight of 26,000 and an isoelectric point of 8.5. Recombinant SEL shared many biological activities with SEs, including superantigenicity, pyrogenicity, enhancement of endotoxin shock, and lethality in rabbits when administered in subcutaneous miniosmotic pumps, but the protein lacked emetic activity. T cells bearing the T-cell receptor ß chain variable regions 5.1, 5.2, 6.7, 16, and 22 were significantly stimulated by recombinant SEL.

* Corresponding author. Mailing address: Department of Microbiology, University of Minnesota Medical School, Mayo Mail Code 196, 420 Delaware St. SE, Minneapolis, MN 55455. Phone: (612) 624-9471. Fax: (612) 626-0623. E-mail: pats{at}lenti.med.umn.edu.

Editor: J. D. Clements

{dagger} Present address: Department of Environmental Engineering Science, California Institute of Technology, Pasadena, CA 91125.

{ddagger} Department of Microbiology, Moyne Institute of Preventive Medicine, University of Dublin, Trinity College, Dublin 2, Ireland.

Infection and Immunity, May 2003, p. 2916-2919, Vol. 71, No. 5
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.5.2916-2919.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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