This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Utaisincharoen, P.
Right arrow Articles by Sirisinha, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Utaisincharoen, P.
Right arrow Articles by Sirisinha, S.

 Previous Article  |  Next Article 

Infection and Immunity, June 2003, p. 3053-3057, Vol. 71, No. 6
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.6.3053-3057.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Involvement of Beta Interferon in Enhancing Inducible Nitric Oxide Synthase Production and Antimicrobial Activity of Burkholderia pseudomallei-Infected Macrophages

P. Utaisincharoen,1,2* N. Anuntagool,1 K. Limposuwan,1 P. Chaisuriya,1 and S. Sirisinha1,2

Laboratory of Immunology, Chulabhorn Research Institute,1 Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand2

Received 9 October 2002/ Returned for modification 22 January 2003/ Accepted 24 February 2003

Burkholderia pseudomallei is the causative agent of melioidosis, a life-threatening disease that affects both humans and animals. This bacterium is able to survive and multiply inside both phagocytic and nonphagocytic cells. We recently reported that mouse macrophages infected with B. pseudomallei fail to produce a significant level of inducible nitric oxide synthase (iNOS), a crucial enzyme needed for the cells to control the intracellular growth of this bacterium. In the present study, we extended our investigation to demonstrate that, unlike other gram-negative bacteria that have been investigated, B. pseudomallei only minimally activates beta interferon (IFN-ß) production; this minimal activation leads to a low level of interferon regulating factor 1 (IRF-1) in the macrophages, in parallel with poor iNOS expression. Adding exogenous IFN-ß to the system could upregulate IRF-1 production, which in turn could enhance iNOS expression in the B. pseudomallei-infected macrophages and lead to suppression of the intracellular growth of this bacterium. Taken together, these results imply that the failure of macrophages to successfully control the growth and survival of intracellular B. pseudomallei is related, at least in part, to the defective production of IFN-ß, which modulates the ability of macrophages to synthesize iNOS.

* Corresponding author. Mailing address: Department of Microbiology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand. Phone: (662) 201-5954. Fax: (662) 201-5972. E-mail: scput{at}mahidol.ac.th.

Editor: J. D. Clements

Infection and Immunity, June 2003, p. 3053-3057, Vol. 71, No. 6
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.6.3053-3057.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Arjcharoen, S., Wikraiphat, C., Pudla, M., Limposuwan, K., Woods, D. E., Sirisinha, S., Utaisincharoen, P. (2007). Fate of a Burkholderia pseudomallei Lipopolysaccharide Mutant in the Mouse Macrophage Cell Line RAW 264.7: Possible Role for the O-Antigenic Polysaccharide Moiety of Lipopolysaccharide in Internalization and Intracellular Survival. Infect. Immun. 75: 4298-4304 [Abstract] [Full Text]  
  • Breitbach, K., Klocke, S., Tschernig, T., van Rooijen, N., Baumann, U., Steinmetz, I. (2006). Role of Inducible Nitric Oxide Synthase and NADPH Oxidase in Early Control of Burkholderia pseudomallei Infection in Mice. Infect. Immun. 74: 6300-6309 [Abstract] [Full Text]  
  • Ekchariyawat, P., Pudla, S., Limposuwan, K., Arjcharoen, S., Sirisinha, S., Utaisincharoen, P. (2005). Burkholderia pseudomallei-Induced Expression of Suppressor of Cytokine Signaling 3 and Cytokine-Inducible Src Homology 2-Containing Protein in Mouse Macrophages: a Possible Mechanism for Suppression of the Response to Gamma Interferon Stimulation. Infect. Immun. 73: 7332-7339 [Abstract] [Full Text]  
  • Moran, J. M., Moxley, M. A., Buller, R. M. L., Corbett, J. A. (2005). Encephalomyocarditis Virus Induces PKR-Independent Mitogen-Activated Protein Kinase Activation in Macrophages. J. Virol. 79: 10226-10236 [Abstract] [Full Text]  
  • Cheng, A. C., Currie, B. J. (2005). Melioidosis: Epidemiology, Pathophysiology, and Management. Clin. Microbiol. Rev. 18: 383-416 [Abstract] [Full Text]  
  • Wongratanacheewin, S., Kespichayawattana, W., Intachote, P., Pichyangkul, S., Sermswan, R. W., Krieg, A. M., Sirisinha, S. (2004). Immunostimulatory CpG Oligodeoxynucleotide Confers Protection in a Murine Model of Infection with Burkholderia pseudomallei. Infect. Immun. 72: 4494-4502 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»