This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ishikawa, S.
Right arrow Articles by Danbara, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ishikawa, S.
Right arrow Articles by Danbara, H.

 Previous Article  |  Next Article 

Infection and Immunity, June 2003, p. 3235-3239, Vol. 71, No. 6
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.6.3235-3239.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Protection against Shiga Toxin 1 Challenge by Immunization of Mice with Purified Mutant Shiga Toxin 1

Satoshi Ishikawa,1 Kazuyoshi Kawahara,2 Yutaka Kagami,2 Yasunori Isshiki,2,{dagger} Aki Kaneko,2,{ddagger} Hidenori Matsui,3 Nobuhiko Okada,1 and Hirofumi Danbara1*

Department of Microbiology, School of Pharmaceutical Sciences,1 Kitasato Institute for Life Science, Kitasato University, Tokyo 108-8641,3 Department of Bacteriology, The Kitasato Institute, Tokyo 108-8642, Japan2

Received 13 November 2002/ Returned for modification 17 January 2003/ Accepted 24 February 2003

Shiga toxin 1 (Stx1) of enterohemorrhagic Escherichia coli O157:H7 was cloned, and four mutant Stx1s were constructed by site-directed mutagenesis with PCR. The wild-type and mutant Stx1s with amino acid replacements at positions 167 and 170 of the A subunit were purified by one-step affinity chromatography with commercially available Globotriose Fractogel, and the mutant Stxs were used for the immunization of mice. The mutant toxins were nontoxic to Vero cells in vitro and to mice in vivo and induced the immunoglobulin G antibody against the wild-type Stx1, which neutralized the cytotoxicity of Stx1. The induced antibody titers depended on the mutation at position 170 of the A subunit. The mice immunized with the mutant Stx1s were protected against a challenge of approximately 100 times the 50% lethal dose of the wild-type Stx1, suggesting that the mutant toxins are good candidates for toxoid vaccines for infection by Stx1-producing E. coli.

* Corresponding author. Mailing address: Department of Microbiology, School of Pharmaceutical Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan. Phone: 81-3-5791-6255. Fax: 81-3-3444-4831. E-mail: danbarah{at}pharm.kitasato-u.ac.jp.

Editor: A. D. O'Brien

{dagger} Present address: Department of Microbiology, School of Pharmaceutical Sciences, Josai University, Sakado-shi, Saitama 350-0295, Japan.

{ddagger} Present address: National Institute of Infectious Diseases, Shinjuku-ku, Tokyo 162-8640, Japan.

Infection and Immunity, June 2003, p. 3235-3239, Vol. 71, No. 6
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.6.3235-3239.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Bentancor, L. V., Bilen, M., Brando, R. J. F., Ramos, M. V., Ferreira, L. C. S., Ghiringhelli, P. D., Palermo, M. S. (2009). A DNA Vaccine Encoding the Enterohemorragic Escherichia coli Shiga-Like Toxin 2 A2 and B Subunits Confers Protective Immunity to Shiga Toxin Challenge in the Murine Model. CVI 16: 712-718 [Abstract] [Full Text]  
  • Agin, T. S., Zhu, C., Johnson, L. A., Thate, T. E., Yang, Z., Boedeker, E. C. (2005). Protection against Hemorrhagic Colitis in an Animal Model by Oral Immunization with Isogeneic Rabbit Enteropathogenic Escherichia coli Attenuated by Truncating Intimin. Infect. Immun. 73: 6608-6619 [Abstract] [Full Text]  
  • Mayr, U. B., Haller, C., Haidinger, W., Atrasheuskaya, A., Bukin, E., Lubitz, W., Ignatyev, G. (2005). Bacterial Ghosts as an Oral Vaccine: a Single Dose of Escherichia coli O157:H7 Bacterial Ghosts Protects Mice against Lethal Challenge. Infect. Immun. 73: 4810-4817 [Abstract] [Full Text]  
  • Talbot, U. M., Paton, J. C., Paton, A. W. (2005). Protective Immunization of Mice with an Active-Site Mutant of Subtilase Cytotoxin of Shiga Toxin-Producing Escherichia coli. Infect. Immun. 73: 4432-4436 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»