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Infection and Immunity, June 2003, p. 3496-3502, Vol. 71, No. 6
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.6.3496-3502.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Helicobacter pylori Activates Toll-Like Receptor 4 Expression in Gastrointestinal Epithelial Cells

Bin Su, Peter J. M. Ceponis, Sylvie Lebel, Hien Huynh, and Philip M. Sherman*

Research Institute, Hospital for Sick Children, Departments of Paediatrics, Physiology, and Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada

Received 3 September 2002/ Returned for modification 19 November 2002/ Accepted 18 March 2003

Helicobacter pylori activates the transcription factor NF-{kappa}B, leading to proinflammatory cytokine production by gastric epithelial cells. However, the receptors for the initial bacterial interaction with host cells which activate downstream signaling events have not been completely defined. Recently, it has been shown that microbial components activate Toll-like receptors (TLRs), thereby leading to AP-1- and NF-{kappa}B-dependent transcription and resulting in the production of proinflammatory cytokines. The aim of this study was to determine whether H. pylori activates TLR4. Reverse transcription-PCR showed that both type I and type II H. pylori clinical isolates induced TLR4 mRNA expression in AGS cells compared with that by uninfected controls. H. pylori upregulated TLR4 protein expression in two gastric epithelial cell lines (AGS and MKN45) and one intestinal epithelial cell line (T84). Monoclonal TLR4 antibody inhibited lipopolysaccharide-induced interleukin-8 secretion from THP-1 macrophages but not from gastric epithelial cells infected with H. pylori. H. pylori demonstrated increased adherence to CHO TLR4-transfected cells compared with that to both CHO TLR2-transfected and nontransfected CHO cells (P < 0.01). These results indicate that H. pylori activates TLR4 expression in epithelial cells and that TLR4 can serve as a receptor for H. pylori binding.

* Corresponding author. Mailing address: Gastroenterology and Nutrition, Room 8409, Hospital for Sick Children, 555 University Ave., Toronto, Ontario, Canada M5G 1X8. Phone: 416-813-7734. Fax: 416-813-6531. E-mail: sherman{at}sickkids.ca.

Editor: V. J. DiRita

Infection and Immunity, June 2003, p. 3496-3502, Vol. 71, No. 6
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.6.3496-3502.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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