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Infection and Immunity, June 2003, p. 3529-3539, Vol. 71, No. 6
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.6.3529-3539.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

pH-Regulated Gene Expression of the Gastric Pathogen Helicobacter pylori

D. Scott Merrell,* Maria L. Goodrich, Glen Otto, Lucy S. Tompkins, and Stanley Falkow

Department of Microbiology and Immunology, Stanford School of Medicine, Stanford, California 94305

Received 31 October 2002/ Returned for modification 28 January 2003/ Accepted 10 March 2003

Colonization by the gastric pathogen Helicobacter pylori has been shown to be intricately linked to the development of gastritis, ulcers, and gastric malignancy. Little is known about mechanisms employed by the bacterium that help it adapt to the hostile environment of the human stomach. In an effort to extend our knowledge of these mechanisms, we utilized spotted-DNA microarrays to characterize the response of H. pylori to low pH. Expression of approximately 7% of the bacterial genome was reproducibly altered by shift to low pH. Analysis of the differentially expressed genes led to the discovery that acid exposure leads to profound changes in motility of H. pylori, as a larger percentage of acid-exposed bacterial cells displayed motility and moved at significantly higher speeds. In contrast to previous publications, we found that expression of the bacterial virulence gene cagA was strongly repressed by acid exposure. Furthermore, this transcriptional repression was reflected at the level of protein accumulation in the H. pylori cell.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Stanford School of Medicine, 299 Campus Dr., Fairchild D051, Stanford, CA 94305. Phone: (650) 725-7161. Fax: (650) 723-1837. E-mail: dmerrell{at}stanford.edu.

Editor: V. J. DiRita

Infection and Immunity, June 2003, p. 3529-3539, Vol. 71, No. 6
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.6.3529-3539.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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