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Infection and Immunity, June 2003, p. 3572-3577, Vol. 71, No. 6
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.6.3572-3577.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Helicobacter-Induced Chronic Active Lymphoid Aggregates Have Characteristics of Tertiary Lymphoid Tissue

Nirah H. Shomer,1,{dagger}* James G. Fox,2 Amy E. Juedes,3,{ddagger} and Nancy H. Ruddle3

Section of Comparative Medicine, Yale University, New Haven, Connecticut 06510,1 Massachusetts Institute of Technology, Division of Comparative Medicine, Cambridge, Massachusetts 02139,2 Department of Epidemiology and Public Health and Section of Immunobiology, Yale University School of Medicine, New Haven, Connecticut 06520-80343

Received 10 December 2002/ Returned for modification 10 February 2003/ Accepted 19 March 2003

Susceptible strains of mice that are naturally or experimentally infected with murine intestinal helicobacter species develop hepatic inflammatory lesions that have previously been described as chronic active hepatitis. The inflammatory infiltrates in some models of chronic autoimmunity or inflammation resemble tertiary lymphoid organs hypothesized to arise by a process termed lymphoid organ neogenesis. To determine whether hepatic inflammation caused by infection with helicobacter could give rise to tertiary lymphoid organs, we used fluorescence-activated cell sorting, immunohistochemistry, and in situ hybridization techniques to identify specific components characteristic of lymphoid organs in liver tissue sections and liver cell suspensions from helicobacter-infected mice. Small venules (high endothelial venules [HEVs]) in inflammatory lesions in Helicobacter species-infected livers were positive for peripheral node addressin. Mucosal addressin cell adhesion molecule also stained HEVs and cells with a staining pattern consistent with scattered stromal cells. The chemokines SLC (CCL 21) and BLC (CXCL13) were present, as were B220-positive B cells and T cells. The latter included a naïve (CD45lo-CD62Lhi) population. These findings suggest that helicobacter-induced chronic active hepatitis arises through the process of lymphoid organ neogenesis.

* Corresponding author. Mailing address: Laboratory Animal Science Center, 715 Albany St., W707, Boston, MA 02118. Phone: (617) 638-4090. Fax: (617) 638-4055. E-mail: nirah{at}bu.edu.

Editor: B. B. Finlay

{dagger} Present address: Boston University, Laboratory Animal Science Center, Boston, MA 02118.

{ddagger} Present address: LaJolla Institute of Allergy and Immunology, San Diego, CA 92121.

Infection and Immunity, June 2003, p. 3572-3577, Vol. 71, No. 6
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.6.3572-3577.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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