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Infection and Immunity, June 2003, p. 3597-3602, Vol. 71, No. 6
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.6.3597-3602.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Comprehensive Analysis of the Factor H Binding Capabilities of Borrelia Species Associated with Lyme Disease: Delineation of Two Distinct Classes of Factor H Binding Proteins

John V. McDowell,1 Jill Wolfgang,2 Emily Tran,1 Michael S. Metts,1 Duncan Hamilton,1 and Richard T. Marconi1,3*

Department of Microbiology and Immunology,1 Division of Infectious Diseases, Department of Internal Medicine,2 Center for the Study of Biological Complexity Medical College of Virginia at Virginia Commonwealth University, Richmond, Virginia 23298-06783

Received 8 November 2002/ Returned for modification 28 January 2003/ Accepted 5 March 2003

Some Lyme disease spirochete isolates can bind complement regulatory protein factor H (fH), a process that may allow evasion of complement-mediated killing. Here we demonstrate significant differences in the fH binding capabilities of species of the Borrelia burgdorferi sensu lato complex. The percentages of B. burgdorferi, B. afzelii, and B. garinii bacteria that bound fH in either enzyme-linked immunosorbent assays or affinity ligand binding immunoblot assays were 100, 83, and 29%, respectively. The fH binding protein profiles were examined and found to exhibit variability among isolates and to form two distinct classes. Differences in fH binding ability may contribute to the differences in pathogenesis and clinical course observed upon infection with different species of the B. burgdorferi sensu lato complex.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Medical College of Virginia at Virginia Commonwealth University, Richmond, VA 23298-0678. Phone: (804) 828-3779. Fax: (804) 828-9946. E-mail: rmarconi{at}hsc.vcu.edu.

Editor: V. J. DiRita

Infection and Immunity, June 2003, p. 3597-3602, Vol. 71, No. 6
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.6.3597-3602.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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