This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bebb, J. R.
Right arrow Articles by Atherton, J. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bebb, J. R.
Right arrow Articles by Atherton, J. C.

 Previous Article  |  Next Article 

Infection and Immunity, June 2003, p. 3623-3627, Vol. 71, No. 6
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.6.3623-3627.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Helicobacter pylori Supernatants Cause Epithelial Cytoskeletal Disruption That Is Bacterial Strain and Epithelial Cell Line Dependent but Not Toxin VacA Dependent

James R. Bebb, Darren P. Letley, Joanne L. Rhead, and John C. Atherton*

Division of Gastroenterology and Institution of Infections, Immunity and Inflammation, University of Nottingham, University Hospital, Nottingham NG7 2VH, United Kingdom

Received 8 November 2002/ Returned for modification 8 January 2003/ Accepted 24 February 2003

We show here that Helicobacter pylori broth culture supernatants disrupt the actin cytoskeleton of epithelial cell lines, leading to cell rounding and apoptosis through anoikis. We demonstrate that there are marked quantitative differences between strains and that there are different cell line sensitivities. By constructing VacA null isogenic mutants, we show that the effect is not due to the vacuolating cytotoxin.

* Corresponding author. Mailing address: Division of Gastroenterology, University Hospital, Nottingham NG7 2UH, United Kingdom. Phone: 44-115-9249924, ext. 41966. Fax: 44-115-9422232. E-mail: john.atherton{at}nottingham.ac.uk.

Editor: A. D. O'Brien

Infection and Immunity, June 2003, p. 3623-3627, Vol. 71, No. 6
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.6.3623-3627.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Argent, R. H., Thomas, R. J., Letley, D. P., Rittig, M. G., Hardie, K. R., Atherton, J. C. (2008). Functional association between the Helicobacter pylori virulence factors VacA and CagA. J Med Microbiol 57: 145-150 [Abstract] [Full Text]  
  • Bebb, J R, Leach, L, Zaitoun, A, Hand, N, Letley, D P, Thomas, R, Atherton, J C (2006). Effects of Helicobacter pylori on the cadherin-catenin complex. J. Clin. Pathol. 59: 1261-1266 [Abstract] [Full Text]  
  • Oliveira, M. J., Costa, A. C., Costa, A. M., Henriques, L., Suriano, G., Atherton, J. C., Machado, J. C., Carneiro, F., Seruca, R., Mareel, M., Leroy, A., Figueiredo, C. (2006). Helicobacter pylori Induces Gastric Epithelial Cell Invasion in a c-Met and Type IV Secretion System-dependent Manner. J. Biol. Chem. 281: 34888-34896 [Abstract] [Full Text]  
  • Boughan, P. K., Argent, R. H., Body-Malapel, M., Park, J.-H., Ewings, K. E., Bowie, A. G., Ong, S. J., Cook, S. J., Sorensen, O. E., Manzo, B. A., Inohara, N., Klein, N. J., Nunez, G., Atherton, J. C., Bajaj-Elliott, M. (2006). Nucleotide-binding Oligomerization Domain-1 and Epidermal Growth Factor Receptor: CRITICAL REGULATORS OF beta-DEFENSINS DURING HELICOBACTER PYLORI INFECTION. J. Biol. Chem. 281: 11637-11648 [Abstract] [Full Text]  
  • Fitchen, N., Letley, D. P, O'Shea, P., Atherton, J. C, Williams, P., Hardie, K. R (2005). All subtypes of the cytotoxin VacA adsorb to the surface of Helicobacter pylori post-secretion. J Med Microbiol 54: 621-630 [Abstract] [Full Text]  
  • Michel, J.-B. (2003). Anoikis in the Cardiovascular System: Known and Unknown Extracellular Mediators. Arterioscler. Thromb. Vasc. Bio. 23: 2146-2154 [Abstract] [Full Text]  
  • Bebb, J R, Letley, D P, Thomas, R J, Aviles, F, Collins, H M, Watson, S A, Hand, N M, Zaitoun, A, Atherton, J C (2003). Helicobacter pylori upregulates matrilysin (MMP-7) in epithelial cells in vivo and in vitro in a Cag dependent manner. Gut 52: 1408-1413 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»