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Infection and Immunity, July 2003, p. 3724-3729, Vol. 71, No. 7
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.7.3724-3729.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Control of the Oxidative Burst of Human Neutrophils by Staphylococcal Leukotoxins

Laboratoire de Physiopathologie et d'Antibiologie des Infections Bactériennes Emergentes et Nosocomiales (UPRES-EA 3432), Institut de Bactériologie de la Faculté de Médecine, Université Louis Pasteur-Hôpitaux Universitaires de Strasbourg, 67000 Strasbourg, France

Received 19 February 2003/ Returned for modification 21 March 2003/ Accepted 4 April 2003

The ability of staphylococcal two-component leukotoxins to induce an oxidative burst and/or to prime human polymorphonuclear cells (PMNs) was studied by using spectrofluorometry or flow cytometry. At sublytic concentrations, the HlgA-HlgB, HlgA-LukF-PV, LukS-PV-LukF-PV, and HlgC-LukF-PV combinations of leukotoxins, but not the LukS-PV-HlgB and HlgC-HlgB combinations, were able to induce H₂O₂ production similar to the H₂O₂ production induced by 1 µM N-formyl-Met-Leu-Phe (fMLP). In addition, when added at sublytic concentrations, all of the leukotoxin combinations primed PMNs for H₂O₂ production induced by fMLP. Leukotoxin activation was dependent on the presence of Ca²⁺ and was inhibited by wortmannin, an inhibitor of phosphatidylinositol 3-kinase, but not by N-methyl-L-arginine, an inhibitor of NO generation, which eliminates the possibility that NO plays a role in the action of leukotoxins. At higher concentrations, all leukotoxins inhibited H₂O₂ production by PMNs activated by fMLP, phorbol 12-myristate 13-acetate (PMA), or the leukotoxins themselves. This inhibition was not related to the pore formation induced by leukotoxins. Intracellular release of H₂O₂ induced by fMLP and PMA was not primed by leukotoxins but was inhibited. It seems that leukotoxin inhibition of H₂O₂ release is independent of pore formation but secondary to an intracellular event, as yet unknown, triggered by leukotoxins.

Editor: J. T. Barbieri

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