This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Midorikawa, K.
Right arrow Articles by Sugai, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Midorikawa, K.
Right arrow Articles by Sugai, M.

 Previous Article  |  Next Article 

Infection and Immunity, July 2003, p. 3730-3739, Vol. 71, No. 7
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.7.3730-3739.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Staphylococcus aureus Susceptibility to Innate Antimicrobial Peptides, ß-Defensins and CAP18, Expressed by Human Keratinocytes

Kazushige Midorikawa,1 Kazuhisa Ouhara,2,3 Hitoshi Komatsuzawa,2* Toshihisa Kawai,4 Sakuo Yamada,5 Tamaki Fujiwara,2 Kenshi Yamazaki,1 Koji Sayama,1 Martin A. Taubman,4 Hidemi Kurihara,3 Koji Hashimoto,1 and Motoyuki Sugai2

Department of Dermatology, Ehime University School of Medicine, Onsen-gun, Ehime 791-0295,1 Department of Bacteriology,2 Department of Periodontology and Endodontology, Hiroshima University Graduate School of Biomedical Sciences, Hiroshima 734-8553,3 Department of Microbiology, Kawasaki Medical School, Matsushima Kurashiki, Okayama 701-0192, Japan,5 Department of Immunology, The Forsyth Institute, Boston, Massachusetts 021154

Received 29 August 2002/ Returned for modification 11 December 2002/ Accepted 25 March 2003

The antimicrobial peptides human ß-defensin-1 (hBD1), hBD2, hBD3, and CAP18 expressed by keratinocytes have been implicated in mediation of the innate defense against bacterial infection. To gain insight into Staphylococcus aureus infection, the susceptibility of S. aureus, including methicillin-resistant S. aureus (MRSA), to these antimicrobial peptides was examined. Based on quantitative PCR, expression of hBD2 mRNA by human keratinocytes was significantly induced by contact with S. aureus, and expression of hBD3 and CAP18 mRNA was slightly induced, while hBD1 mRNA was constitutively expressed irrespective of the presence of S. aureus. Ten clinical S. aureus isolates, including five MRSA isolates, induced various levels of expression of hBD2, hBD3, and CAP18 mRNA by human kertinocytes. The activities of hBD3 and CAP18 against S. aureus were found to be greater than those of hBD1 and hBD2. A total of 44 S. aureus clinical isolates, including 22 MRSA strains, were tested for susceptibility to hBD3 and CAP18. Twelve (55%) and 13 (59%) of the MRSA strains exhibited more than 20% survival in the presence of hBD3 (1 µg/ml) and CAP18 (0.5 µg/ml), respectively. However, only three (13%) and two (9%) of the methicillin-sensitive S. aureus isolates exhibited more than 20% survival with hBD3 and CAP18, respectively, suggesting that MRSA is more resistant to these peptides. A synergistic antimicrobial effect between suboptimal doses of methicillin and either hBD3 or CAP18 was observed with 10 MRSA strains. Furthermore, of several genes associated with methicillin resistance, inactivation of the fmtC gene in MRSA strain COL increased susceptibility to the antimicrobial effect mediated by hBD3 or CAP18.

* Corresponding author. Mailing address: Department of Microbiology, Hiroshima University Faculty of Dentistry, Kasumi 1-2-3, Minami-ku, Hiroshima City, Hiroshima 734-8553, Japan. Phone: 81 82 257 5636. Fax: 81 82 257 5639. E-mail: hkomatsu{at}hiroshima-u.ac.jp.

Editor: V. J. DiRita

Infection and Immunity, July 2003, p. 3730-3739, Vol. 71, No. 7
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.7.3730-3739.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Liu, N., Kaplan, A.T., Low, J., Nguyen, L., Liu, G.Y., Equils, O., Hewison, M. (2009). Vitamin D Induces Innate Antibacterial Responses in Human Trophoblasts via an Intracrine Pathway. Biol. Reprod. 80: 398-406 [Abstract] [Full Text]  
  • Diamond, G., Beckloff, N., Ryan, L.K. (2008). Host Defense Peptides in the Oral Cavity and the Lung: Similarities and Differences. JDR 87: 915-927 [Abstract] [Full Text]  
  • Ouhara, K., Komatsuzawa, H., Kawai, T., Nishi, H., Fujiwara, T., Fujiue, Y., Kuwabara, M., Sayama, K., Hashimoto, K., Sugai, M. (2008). Increased resistance to cationic antimicrobial peptide LL-37 in methicillin-resistant strains of Staphylococcus aureus. J Antimicrob Chemother 61: 1266-1269 [Abstract] [Full Text]  
  • Harder, J., Glaser, R., Schroder, J.-M. (2007). Review: Human antimicrobial proteins effectors of innate immunity. Innate Immunity 13: 317-338 [Abstract]  
  • Tohyama, M., Sayama, K., Komatsuzawa, H., Hanakawa, Y., Shirakata, Y., Dai, X., Yang, L., Tokumaru, S., Nagai, H., Hirakawa, S., Sugai, M., Hashimoto, K. (2007). CXCL16 is a novel mediator of the innate immunity of epidermal keratinocytes. Int Immunol 19: 1095-1102 [Abstract] [Full Text]  
  • Menzies, B. E., Kenoyer, A. (2006). Signal Transduction and Nuclear Responses in Staphylococcus aureus- Induced Expression of Human {beta}-Defensin 3 in Skin Keratinocytes. Infect. Immun. 74: 6847-6854 [Abstract] [Full Text]  
  • Ouhara, K., Komatsuzawa, H., Shiba, H., Uchida, Y., Kawai, T., Sayama, K., Hashimoto, K., Taubman, M. A., Kurihara, H., Sugai, M. (2006). Actinobacillus actinomycetemcomitans Outer Membrane Protein 100 Triggers Innate Immunity and Production of {beta}-Defensin and the 18-Kilodalton Cationic Antimicrobial Protein through the Fibronectin-Integrin Pathway in Human Gingival Epithelial Cells. Infect. Immun. 74: 5211-5220 [Abstract] [Full Text]  
  • Friedman, L., Alder, J. D., Silverman, J. A. (2006). Genetic Changes That Correlate with Reduced Susceptibility to Daptomycin in Staphylococcus aureus.. Antimicrob. Agents Chemother. 50: 2137-2145 [Abstract] [Full Text]  
  • Weidenmaier, C., Peschel, A., Kempf, V. A. J., Lucindo, N., Yeaman, M. R., Bayer, A. S. (2005). DltABCD- and MprF-Mediated Cell Envelope Modifications of Staphylococcus aureus Confer Resistance to Platelet Microbicidal Proteins and Contribute to Virulence in a Rabbit Endocarditis Model. Infect. Immun. 73: 8033-8038 [Abstract] [Full Text]  
  • Tokumaru, S., Sayama, K., Shirakata, Y., Komatsuzawa, H., Ouhara, K., Hanakawa, Y., Yahata, Y., Dai, X., Tohyama, M., Nagai, H., Yang, L., Higashiyama, S., Yoshimura, A., Sugai, M., Hashimoto, K. (2005). Induction of Keratinocyte Migration via Transactivation of the Epidermal Growth Factor Receptor by the Antimicrobial Peptide LL-37. J. Immunol. 175: 4662-4668 [Abstract] [Full Text]  
  • Menzies, B. E., Kenoyer, A. (2005). Staphylococcus aureus Infection of Epidermal Keratinocytes Promotes Expression of Innate Antimicrobial Peptides. Infect. Immun. 73: 5241-5244 [Abstract] [Full Text]  
  • McMichael, J. W., Maxwell, A. I., Hayashi, K., Taylor, K., Wallace, W. A., Govan, J. R., Dorin, J. R., Sallenave, J.-M. (2005). Antimicrobial Activity of Murine Lung Cells against Staphylococcus aureus Is Increased In Vitro and In Vivo after Elafin Gene Transfer. Infect. Immun. 73: 3609-3617 [Abstract] [Full Text]  
  • Ouhara, K., Komatsuzawa, H., Yamada, S., Shiba, H., Fujiwara, T., Ohara, M., Sayama, K., Hashimoto, K., Kurihara, H., Sugai, M. (2005). Susceptibilities of periodontopathogenic and cariogenic bacteria to antibacterial peptides, {beta}-defensins and LL37, produced by human epithelial cells. J Antimicrob Chemother 55: 888-896 [Abstract] [Full Text]  
  • Nishi, H., Komatsuzawa, H., Fujiwara, T., McCallum, N., Sugai, M. (2004). Reduced Content of Lysyl-Phosphatidylglycerol in the Cytoplasmic Membrane Affects Susceptibility to Moenomycin, as Well as Vancomycin, Gentamicin, and Antimicrobial Peptides, in Staphylococcus aureus. Antimicrob. Agents Chemother. 48: 4800-4807 [Abstract] [Full Text]  
  • Shi, L., Takahashi, K., Dundee, J., Shahroor-Karni, S., Thiel, S., Jensenius, J. C., Gad, F., Hamblin, M. R., Sastry, K. N., Ezekowitz, R. A. B. (2004). Mannose-binding Lectin-deficient Mice Are Susceptible to Infection with Staphylococcus aureus. JEM 199: 1379-1390 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»