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Infection and Immunity, July 2003, p. 3837-3843, Vol. 71, No. 7
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.7.3837-3843.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Antigenic Properties of HpaA and Omp18, Two Outer Membrane Proteins of Helicobacter pylori

Petra Voland,1 Nadia Hafsi,1 Marco Zeitner,1 Stephanie Laforsch,1 Hermann Wagner,2 and Christian Prinz1*

Department of Medicine and Gastroenterology,1 Department of Microbiology and Immunology, Technical University of Munich, Munich, Germany2

Received 20 December 2002/ Returned for modification 20 February 2003/ Accepted 20 March 2003

Outer membrane proteins (OMPs) are incorporated into the outer plasma membrane of Helicobacter pylori and are important for, e.g., ion transport, adherence, structural and osmotic stability, and bacterial virulence but may also be antigenic due to their surface exposure. Previous proteome-based approaches with H. pylori lysates determined a strong serological reaction towards two H. pylori OMPs, HpaA (TIGR HP0797) and Omp18 (TIGR HP1125). PCR was used to detect DNA encoding the two proteins, and a positive signal was found in all H. pylori strains tested. Proteins were cloned and expressed in the human kidney cell line HK293 with the QiaExpressionist system with a C-terminal His tag. Only sera from infected persons showed a positive reaction with the recombinant proteins. Recombinant HpaA (rHpaA) and rOmp18 were incubated with human peripheral blood mononuclear cells and induced secretion of interleukin-12 (IL-12) and IL-10 from these cells. To determine the effect on antigen-presenting cells, human blood monocytic and dendritic cells (DCs) were isolated by magnetic cell separation. rOmp18 and rHpaA strongly stimulated major histocompatibility class II and CD83 expression 7- to 10-fold on isolated DCs. rHpaA and rOmp18 failed to stimulate IL-8 secretion from monocytes but increased secretion of IL-12 and IL-10 from DCs significantly. In summary, HpaA and Omp18 are recognized by human dendritic cells and induce their maturation as well as antigen presentation. HpaA and Omp18 of H. pylori thereby appear to have a specific antigenic potential in humans.

* Corresponding author. Mailing address: Department of Medicine II, Technical University of Munich, Ismaningerstr. 22, 81675 Munich, Germany. Phone: 49-89-4140-4073. Fax: 49-89-4140-7366. E-mail: christian.prinz{at}lrz.tum.de.

Editor: F. C. Fang

Infection and Immunity, July 2003, p. 3837-3843, Vol. 71, No. 7
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.7.3837-3843.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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