This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Capozzo, A. V. E.
Right arrow Articles by Palermo, M. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Capozzo, A. V. E.
Right arrow Articles by Palermo, M. S.

 Previous Article  |  Next Article 

Infection and Immunity, July 2003, p. 3971-3978, Vol. 71, No. 7
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.7.3971-3978.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Development of DNA Vaccines against Hemolytic-Uremic Syndrome in a Murine Model

Alejandra V. E. Capozzo,1 Virginia Pistone Creydt,2 Graciela Dran,1 Gabriela Fernández,1 Sonia Gómez,1 Leticia V. Bentancor,1 Carolina Rubel,1 Cristina Ibarra,2 Martín Isturiz,1 and Marina S. Palermo1*

Instituto de Investigaciones Hematológicas, Academia Nacional de Medicina,1 Departamento de Fisiología, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina2

Received 9 October 2002/ Returned for modification 26 November 2002/ Accepted 12 March 2003

Shiga toxin type 2 (Stx2) produced by Escherichia coli O:157H7 can cause hemolytic-uremic syndrome in children, a disease for which there is neither a vaccine nor an effective treatment. This toxin consists of an enzymatically active A subunit and a pentameric B subunit responsible for the toxin binding to host cells, and also found to be immunogenic in rabbits. In this study we developed eukaryotic plasmids expressing the B subunit gene of Stx2 (pStx2B) and the B subunit plus the gene coding for the A subunit with an active-site deletion (pStx2{Delta}A). Transfection of eukaryotic cells with these plasmids produced proteins of the expected molecular weight which reacted with specific monoclonal antibodies. Newborn and adult BALB/c mice immunized with two intramuscular injections of each plasmid, either alone or together with the same vector expressing the granulocyte and monocyte colony-stimulating factor (pGM-CSF), elicited a specific Th1-biased humoral response. The effect of pGM-CSF as an adjuvant plasmid was particularly notable in newborn mice and in pStx2B-vaccinated adult mice. Stx2-neutralizing activity, evaluated in vitro on VERO cell monolayers, correlated with in vivo protection. This is the first report using plasmids to induce a neutralizing humoral immune response against the Stx2.

* Corresponding author. Mailing address: Div. Inmunología, Instituto de Investigaciones Hematológicas, Academia Nacional de Medicina, P. de Melo 3081 (1425), Buenos Aires, Argentina. Phone: 5411-4805-5695. Fax: 5411-4803-9475. E-mail: mspalermo{at}hematologia.anm.edu.ar.

Editor: A. D. O'Brien

Infection and Immunity, July 2003, p. 3971-3978, Vol. 71, No. 7
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.7.3971-3978.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Bentancor, L. V., Bilen, M., Brando, R. J. F., Ramos, M. V., Ferreira, L. C. S., Ghiringhelli, P. D., Palermo, M. S. (2009). A DNA Vaccine Encoding the Enterohemorragic Escherichia coli Shiga-Like Toxin 2 A2 and B Subunits Confers Protective Immunity to Shiga Toxin Challenge in the Murine Model. CVI 16: 712-718 [Abstract] [Full Text]  
  • Agin, T. S., Zhu, C., Johnson, L. A., Thate, T. E., Yang, Z., Boedeker, E. C. (2005). Protection against Hemorrhagic Colitis in an Animal Model by Oral Immunization with Isogeneic Rabbit Enteropathogenic Escherichia coli Attenuated by Truncating Intimin. Infect. Immun. 73: 6608-6619 [Abstract] [Full Text]  
  • Capozzo, A. V. E., Cuberos, L., Levine, M. M., Pasetti, M. F. (2004). Mucosally Delivered Salmonella Live Vector Vaccines Elicit Potent Immune Responses against a Foreign Antigen in Neonatal Mice Born to Naive and Immune Mothers. Infect. Immun. 72: 4637-4646 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»