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Infection and Immunity, July 2003, p. 4034-4039, Vol. 71, No. 7
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.7.4034-4039.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Recombinant Soluble CD14 Reduces Severity of Intramammary Infection by Escherichia coli

Jai-Wei Lee,1 Max J. Paape,2 Theodore H. Elsasser,3 and Xin Zhao1*

Department of Animal Science, McGill University, Ste-Anne-de-Bellevue, Quebec H9X 3V9, Canada,1 Immunology and Disease Resistance Laboratory,2 Growth Biology Laboratory, USDA Agricultural Research Service, Beltsville, Maryland 209013

Received 17 January 2003/ Returned for modification 20 March 2003/ Accepted 23 April 2003

The interaction among gram-negative bacteria, the innate immune system, and soluble CD14 (sCD14) has not been well documented. The effect of recombinant bovine sCD14 (rbosCD14) on milk somatic cell count (SCC), bacterial clearance, and cytokine production was investigated by using a bovine intramammary Escherichia coli infection model. We first determined whether rbosCD14 would increase the SCC during a lipopolysaccharide (LPS) challenge. Three quarters of each of six healthy lactating cows were injected with either 0.3 µg of LPS, 0.3 µg of LPS plus 100 µg of rbosCD14, or saline. In comparison with quarters injected with LPS alone, the SCC was twofold higher (P < 0.05) in quarters injected with LPS plus rbosCD14 after the challenge. We therefore hypothesized that when E. coli bacteria invade the mammary gland, sCD14 in milk would interact with LPS and rapidly recruit neutrophils from the blood to eliminate the bacteria before establishment of infection. To test this hypothesis, two quarters of each of nine healthy cows were challenged with either 50 CFU of E. coli plus saline or 50 CFU of E. coli plus 100 µg of rbosCD14. Quarters challenged with E. coli plus rbosCD14 had a more rapid recruitment of neutrophils, which was accompanied by a faster clearance of bacteria, lower concentrations of tumor necrosis factor alpha and interleukin-8 in milk, and milder clinical symptoms, than challenged quarters injected with saline. Results indicate that increasing the concentration of sCD14 in milk may be a potential strategy with which to prevent or reduce the severity of infection by coliform bacteria.

* Corresponding author. Mailing address: Department of Animal Science, McGill University, Ste-Anne-de-Bellevue, QC H9X 3V9, Canada. Phone: (514) 398-7975. Fax: (514) 398-7964. E-mail: Zhao{at}macdonald.mcgill.ca.

Editor: B. B. Finlay

Infection and Immunity, July 2003, p. 4034-4039, Vol. 71, No. 7
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.7.4034-4039.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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