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Infection and Immunity, July 2003, p. 4144-4150, Vol. 71, No. 7
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.7.4144-4150.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Pseudomonas aeruginosa ExoU, a Toxin Transported by the Type III Secretion System, Kills Saccharomyces cerevisiae

Shira D. P. Rabin1 and Alan R. Hauser1,2*

Departments of Microbiology/Immunology,1 Medicine, Northwestern University, Chicago, Illinois 606112

Received 24 February 2003/ Accepted 16 April 2003

ExoU, a protein transported by the type III secretion system of Pseudomonas aeruginosa, is an important cytotoxin, though its mechanism of action is unclear. Here we show that the intracellular expression of ExoU is cytotoxic to Saccharomyces cerevisiae. Furthermore, internal amino- and carboxyl-terminal deletions confirmed that regions of ExoU previously shown to be essential for killing mammalian cells were also required for killing yeast cells. These findings indicate that S. cerevisiae is a useful model organism for the study of ExoU.

* Corresponding author. Mailing address: Department of Microbiology/Immunology, Northwestern University, 303 East Chicago Ave., Searle 6-495, Chicago, IL 60611. Phone: (312) 503-1044. Fax: (312) 503-1339. E-mail: ahauser{at}northwestern.edu.

Editor: V. J. DiRita

Infection and Immunity, July 2003, p. 4144-4150, Vol. 71, No. 7
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.7.4144-4150.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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