This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Falzano, L. Articles by Fiorentini, C. Search for Related Content PubMed PubMed Citation Articles by Falzano, L. Articles by Fiorentini, C.

 Previous Article  |  Next Article 

Infection and Immunity, July 2003, p. 4178-4181, Vol. 71, No. 7
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.7.4178-4181.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Cytotoxic Necrotizing Factor 1 Enhances Reactive Oxygen Species-Dependent Transcription and Secretion of Proinflammatory Cytokines in Human Uroepithelial Cells

Department of Ultrastructures,¹ Department of Immunology, Istituto Superiore di Sanità, 00161 Rome, Italy²

Received 16 December 2002/ Returned for modification 27 January 2003/ Accepted 28 March 2003

Uropathogenic Escherichia coli strains frequently produce a Rho-activating protein toxin named cytotoxic necrotizing factor type 1 (CNF1). We herein report that CNF1 promotes transcription and release of tumor necrosis factor alpha, gamma interferon, interleukin-6 (IL-6), and IL-8 proinflammatory cytokines and increases the production of reactive oxygen species (ROS) in uroepithelial T24 cells. The antioxidant N-acetyl-L-cysteine counteracts these phenomena, a fact which suggests a role for ROS-mediated signaling in CNF1-induced proinflammatory cytokine production.

Editor: F. C. Fang

This article has been cited by other articles:

• Miraglia, A. G., Travaglione, S., Meschini, S., Falzano, L., Matarrese, P., Quaranta, M. G., Viora, M., Fiorentini, C., Fabbri, A. (2007). Cytotoxic Necrotizing Factor 1 Prevents Apoptosis via the Akt/I{kappa}B Kinase Pathway: Role of Nuclear Factor-{kappa}B and Bcl-2. Mol. Biol. Cell 18: 2735-2744 [Abstract] [Full Text]  
• Falzano, L., Filippini, P., Travaglione, S., Miraglia, A. G., Fabbri, A., Fiorentini, C. (2006). Escherichia coli Cytotoxic Necrotizing Factor 1 Blocks Cell Cycle G2/M Transition in Uroepithelial Cells. Infect. Immun. 74: 3765-3772 [Abstract] [Full Text]  
• Brest, P., Turchi, L., Le'Negrate, G., Berto, F., Moreilhon, C., Mari, B., Ponzio, G., Hofman, P. (2004). Escherichia coli Cytotoxic Necrotizing Factor 1 Inhibits Intestinal Epithelial Wound Healing In Vitro after Mechanical Injury. Infect. Immun. 72: 5733-5740 [Abstract] [Full Text]