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Infection and Immunity, July 2003, p. 4190-4192, Vol. 71, No. 7
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.7.4190-4192.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Vaccine Efficacy of a Lysine Auxotroph of Mycobacterium tuberculosis

Martin S. Pavelka Jr.,^1, Bing Chen,² Cynthia L. Kelley,³ Frank M. Collins,³ and William R. Jacobs Jr.^2*

Howard Hughes Medical Institute,² Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York 10461,¹ Laboratory of Mycobacterial Diseases, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892³

Received 17 May 2002/ Returned for modification 20 August 2002/ Accepted 26 March 2003

The in vivo growth phenotype and vaccine efficacy of a lysine auxotrophic mutant of Mycobacterium tuberculosis strain H37Rv are described. An immunization experiment using a mouse model with an aerosol challenge showed that two doses of the M. tuberculosis mutant were required to generate protection equivalent to that of the Mycobacterium bovis BCG vaccine.

Editor: S. H. E. Kaufmann

Present address: Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642.

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