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Infection and Immunity, July 2003, p. 4190-4192, Vol. 71, No. 7
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.7.4190-4192.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Vaccine Efficacy of a Lysine Auxotroph of Mycobacterium tuberculosis

Martin S. Pavelka Jr.,1,{dagger} Bing Chen,2 Cynthia L. Kelley,3 Frank M. Collins,3 and William R. Jacobs Jr.2*

Howard Hughes Medical Institute,2 Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York 10461,1 Laboratory of Mycobacterial Diseases, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 208923

Received 17 May 2002/ Returned for modification 20 August 2002/ Accepted 26 March 2003

The in vivo growth phenotype and vaccine efficacy of a lysine auxotrophic mutant of Mycobacterium tuberculosis strain H37Rv are described. An immunization experiment using a mouse model with an aerosol challenge showed that two doses of the M. tuberculosis mutant were required to generate protection equivalent to that of the Mycobacterium bovis BCG vaccine.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Albert Einstein College of Medicine, 1300 Morris Park Ave., Bronx, NY 10461. Phone: (718) 430-2888. Fax: (718) 518-0366. E-mail: jacobs{at}aecom.yu.edu.

Editor: S. H. E. Kaufmann

{dagger} Present address: Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642.

Infection and Immunity, July 2003, p. 4190-4192, Vol. 71, No. 7
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.7.4190-4192.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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