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Infection and Immunity, July 2003, p. 4206-4211, Vol. 71, No. 7
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.7.4206-4211.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Mutation of sarA in Staphylococcus aureus Limits Biofilm Formation

Karen E. Beenken, Jon S. Blevins,{dagger} and Mark S. Smeltzer*

Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205

Received 8 January 2003/ Returned for modification 5 March 2003/ Accepted 30 April 2003

Mutation of sarA resulted in a reduced capacity to form a biofilm in six of the eight Staphylococcus aureus strains we tested (UAMS-1, UAMS-601, SA113, SC-01, S6C, and DB). The exceptions were Newman, which formed a poor biofilm under all conditions, and RN6390, which consistently formed a biofilm only after mutation of agr. Mutation of agr in other strains had little impact on biofilm formation. In every strain other than Newman, including RN6390, simultaneous mutation of sarA and agr resulted in a phenotype like that observed with the sarA mutants. Complementation studies using a sarA clone confirmed that the defect in biofilm formation was due to the sarA mutation.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Mail Slot 511, University of Arkansas for Medical Sciences, Little Rock, AR 72205. Phone: (501) 686-7958. Fax: (501) 686-5359. E-mail: smeltzermarks{at}uams.edu.

Editor: V. J. DiRita

{dagger} Present address: Department of Microbiology, University of Texas Southwestern Medical Center at Dallas, Dallas, Tex.

Infection and Immunity, July 2003, p. 4206-4211, Vol. 71, No. 7
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.7.4206-4211.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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