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Infection and Immunity, August 2003, p. 4463-4471, Vol. 71, No. 8
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.8.4463-4471.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Identification of the agr Locus of Listeria monocytogenes: Role in Bacterial Virulence

Nicolas Autret, Catherine Raynaud, Iharilalao Dubail, Patrick Berche, and Alain Charbit*

INSERM U-570, CHU Necker-Enfants Malades, 75730 Paris Cedex 15, France

Received 17 March 2003/ Returned for modification 2 May 2003/ Accepted 16 May 2003

Listeria monocytogenes is a gram-positive facultative intracellular food-borne pathogen that can cause severe infections in humans and animals. We have recently adapted signature-tagged transposon mutagenesis (STM) to identify genes involved in the virulence of L. monocytogenes. A new round of STM allowed us to identify a new locus encoding a protein homologous to AgrA, the well-studied response regulator of Staphylococcus aureus and part of a two-component system involved in bacterial virulence. The production of several secreted proteins was modified in the agrA mutant of L. monocytogenes grown in broth, indicating that the agr locus influenced protein secretion. Inactivation of agrA did not affect the ability of the pathogen to invade and multiply in cells in vitro. However, the virulence of the agrA mutant was attenuated in the mouse (a 10-fold increase in the 50% lethal dose by the intravenous route), demonstrating for the first time a role for the agr locus in the virulence of L. monocytogenes.

* Corresponding author. Mailing address: Faculté de Médecine Necker, 156, rue de Vaugirard, 75730 Paris Cedex 15, France. Phone: 33 1 40 61 53 76. Fax: 33 1 40 61 55 92. E-mail: charbit{at}necker.fr.

Editor: J. T. Barbieri

Infection and Immunity, August 2003, p. 4463-4471, Vol. 71, No. 8
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.8.4463-4471.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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