This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kyd, J. M.
Right arrow Articles by Bakaletz, L. O.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kyd, J. M.
Right arrow Articles by Bakaletz, L. O.

 Previous Article  |  Next Article 

Infection and Immunity, August 2003, p. 4691-4699, Vol. 71, No. 8
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.8.4691-4699.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Efficacy of the 26-Kilodalton Outer Membrane Protein and Two P5 Fimbrin-Derived Immunogens To Induce Clearance of Nontypeable Haemophilus influenzae from the Rat Middle Ear and Lungs as Well as from the Chinchilla Middle Ear and Nasopharynx

Jennelle M. Kyd,1* Allan W. Cripps,1 Laura A. Novotny,1 and Lauren O. Bakaletz2*

Division of Science and Design, Gadi Research Centre, University of Canberra, Canberra, Australia,1 Department of Pediatrics, Division of Molecular Medicine, The Ohio State University College of Medicine and Public Health, Columbus, Ohio2

Received 13 February 2003/ Returned for modification 18 March 2003/ Accepted 13 May 2003

The rat middle ear and lung clearance model has been used to show that the nontypeable Haemophilus influenzae 26-kDa outer membrane protein OMP26 is highly efficacious as a mucosal immunogen, inducing significantly enhanced clearance in immunized rats upon direct challenge of these two anatomic sites. Similarly, the chinchilla model of middle ear and nasopharyngeal clearance has been used to show that two P5 fimbrin adhesin-derived immunogens, LB1 and lipoprotein D (LPD)-LB1(f)2,1,3, are highly efficacious as parenteral immunogens. Both induced significantly augmented clearance of nontypeable H. influenzae upon challenge of these sites. Here, these three nontypeable H. influenzae immunogens in addition to six bovine serum albumin and keyhole limpet hemocyanin conjugates of the synthetic peptide LB1(f) were assayed for relative efficacy in the reciprocal rodent model system. OMP26 was assayed in the chinchilla host by a parenteral immunization route, with clearance of the middle ear and nasopharynx used as outcome measures. Both LB1 and LPD-LB1(f)2,1,3 were assayed in the rat host with a mucosal immunization route and clearance of nontypeable H. influenzae from the lungs and middle ears as outcome measures. Both of the immunogens were found to induce a high-titered and specific immune responses in the heterologous host system. Moreover, each was found to be highly efficacious in the reciprocal host system, providing strong support for the continued development and inclusion of both OMP26 and P5 fimbrin-derived peptides as candidate vaccine antigens directed at otitis media caused by nontypeable H. influenzae.

* Corresponding authors. Mailing address for Jennelle M. Kyd: Division of Health, Design & Science, University of Canberra, Canberra 2601, Australia. Phone: 61-2-6201 2160. Fax: 61-2-6201 2461. E-mail: jennelle.kyd{at}canberra.edu.au. Mailing address for Lauren O. Bakaletz: Department of Pediatrics, Division of Molecular Medicine, The Ohio State University College of Medicine & Public Health, Children's Research Institute W302, 700 Children's Drive, Columbus, OH 43205-2696. Phone: (614) 722-2915. Fax: (614) 722-2007. E-mail: bakaletl{at}pediatrics.ohio-state.edu.

Editor: B. B. Finlay

Infection and Immunity, August 2003, p. 4691-4699, Vol. 71, No. 8
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.8.4691-4699.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Pang, B., Winn, D., Johnson, R., Hong, W., West-Barnette, S., Kock, N., Swords, W. E. (2008). Lipooligosaccharides Containing Phosphorylcholine Delay Pulmonary Clearance of Nontypeable Haemophilus influenzae. Infect. Immun. 76: 2037-2043 [Abstract] [Full Text]  
  • Novotny, L. A., Partida-Sanchez, S., Munson, R. S. Jr., Bakaletz, L. O. (2008). Differential Uptake and Processing of a Haemophilus influenzae P5-Derived Immunogen by Chinchilla Dendritic Cells. Infect. Immun. 76: 967-977 [Abstract] [Full Text]  
  • Bookwalter, J. E., Jurcisek, J. A., Gray-Owen, S. D., Fernandez, S., McGillivary, G., Bakaletz, L. O. (2008). A Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 Homologue Plays a Pivotal Role in Nontypeable Haemophilus influenzae Colonization of the Chinchilla Nasopharynx via the Outer Membrane Protein P5-Homologous Adhesin. Infect. Immun. 76: 48-55 [Abstract] [Full Text]  
  • Winter, L. E., Barenkamp, S. J. (2006). Antibodies Specific for the High-Molecular-Weight Adhesion Proteins of Nontypeable Haemophilus influenzae Are Opsonophagocytic for both Homologous and Heterologous Strains. CVI 13: 1333-1342 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»