This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Foster, N. Articles by Barrow, P. A. Search for Related Content PubMed PubMed Citation Articles by Foster, N. Articles by Barrow, P. A.

 Previous Article  |  Next Article 

Infection and Immunity, August 2003, p. 4733-4741, Vol. 71, No. 8
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.8.4733-4741.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Induction of Antimicrobial Pathways during Early-Phase Immune Response to Salmonella spp. in Murine Macrophages: Gamma Interferon (IFN-) and Upregulation of IFN- Receptor Alpha Expression Are Required for NADPH Phagocytic Oxidase gp91-Stimulated Oxidative Burst and Control of Virulent Salmonella spp.

Division of Environmental Microbiology, Institute for Animal Health, Compton Laboratory, Newbury, Berkshire RG20 7NN, United Kingdom

Received 4 December 2002/ Returned for modification 11 February 2003/ Accepted 1 May 2003

The effect of gamma interferon (IFN-) on elevation of reactive oxygen species and the viability of virulent wild-type and avirulent mutants of Salmonella enterica serovar Typhimurium and S. enterica serovar Infantis was studied in a murine macrophage cell line (J774.2 cells). S. enterica serovar Typhimurium 14028 phoP and a rough lipopolysaccharide mutant of S. enterica serovar Infantis 1326/28 (^r) (avirulent mutants) induced NADPH phagocytic oxidase gp91 (gp91^phox) activity and a significant (P < 0.05) elevation of reactive oxygen species within 12 h without coculture with IFN-. This coincided with reduced survival of S. enterica serovar Typhimurium14028 phoP or stasis of S. enterica serovar Infantis ^r. Fluorometric studies indicated that expression of IFN- on infected J774.2 cells was not significantly (P > 0.05) elevated. However, studies with the virulent S. enterica serovar Typhimurium strains showed that a comparable level of control of bacterial numbers could only be achieved by coculture with IFN-. This coincided with significant upregulation of IFN- receptor alpha expression on the surface of J774.2 cells and was completely abolished by N-acetyl-L-cysteine captopril (an inhibitor of reactive oxygen species). Delay in reactive oxygen species induction due to a requirement for IFN- and upregulation of IFN- receptor alpha in macrophages infected with virulent salmonellae may result in greater dissemination of virulent salmonellae in host tissue.

Editor: B. B. Finlay

This article has been cited by other articles:

• Lahiri, A., Das, P., Chakravortty, D. (2008). The LysR-type transcriptional regulator Hrg counteracts phagocyte oxidative burst and imparts survival advantage to Salmonella enterica serovar Typhimurium. Microbiology 154: 2837-2846 [Abstract] [Full Text]  
• Kannan, S., Audet, A., Huang, H., Chen, L.-j., Wu, M. (2008). Cholesterol-Rich Membrane Rafts and Lyn Are Involved in Phagocytosis during Pseudomonas aeruginosa Infection. J. Immunol. 180: 2396-2408 [Abstract] [Full Text]  
• Singhal, A., Jaiswal, A., Arora, V. K., Prasad, H. K. (2007). Modulation of Gamma Interferon Receptor 1 by Mycobacterium tuberculosis: a Potential Immune Response Evasive Mechanism. Infect. Immun. 75: 2500-2510 [Abstract] [Full Text]  
• Lippuner, N., Morell, B., Schaffner, A., Schaer, D. J. (2007). Proteinase-activated receptors induce nonoxidative, antimicrobial peptides and increased antimicrobial activity in human mononuclear phagocytes. J. Leukoc. Biol. 81: 465-473 [Abstract] [Full Text]  
• Daniel, D. S., Dai, G., Singh, C. R., Lindsey, D. R., Smith, A. K., Dhandayuthapani, S., Hunter, R. L. Jr, Jagannath, C. (2006). The Reduced Bactericidal Function of Complement C5-Deficient Murine Macrophages Is Associated with Defects in the Synthesis and Delivery of Reactive Oxygen Radicals to Mycobacterial Phagosomes. J. Immunol. 177: 4688-4698 [Abstract] [Full Text]  
• Foster, N., Cheetham, J., Taylor, J.J., Preshaw, P.M. (2005). VIP Inhibits Porphyromonas gingivalis LPS-induced Immune Responses in Human Monocytes. J. Dent. Res. 84: 999-1004 [Abstract] [Full Text]  
• Rosales-Reyes, R., Alpuche-Aranda, C., Ramirez-Aguilar, M. d. l. L., Castro-Eguiluz, A. D., Ortiz-Navarrete, V. (2005). Survival of Salmonella enterica Serovar Typhimurium within Late Endosomal-Lysosomal Compartments of B Lymphocytes Is Associated with the Inability To Use the Vacuolar Alternative Major Histocompatibility Complex Class I Antigen-Processing Pathway. Infect. Immun. 73: 3937-3944 [Abstract] [Full Text]  
• Gordon, M. A., Jack, D. L., Dockrell, D. H., Lee, M. E., Read, R. C. (2005). Gamma Interferon Enhances Internalization and Early Nonoxidative Killing of Salmonella enterica Serovar Typhimurium by Human Macrophages and Modifies Cytokine Responses. Infect. Immun. 73: 3445-3452 [Abstract] [Full Text]