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Infection and Immunity, August 2003, p. 4767-4771, Vol. 71, No. 8
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.8.4767-4771.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Nonspecific Immunoglobulin M Binding and Chondroitin Sulfate A Binding Are Linked Phenotypes of Plasmodium falciparum Isolates Implicated in Malaria during Pregnancy

Alison M. Creasey,1* Trine Staalsoe,2 Ahmed Raza,1 David E. Arnot,1 and J. Alexandra Rowe1

Institute of Cell, Animal and Population Biology, University of Edinburgh, Edinburgh EH9 3JT, United Kingdom,1 Department of Infectious Diseases M76411, Rigshospitalet, 2000 Copenhagen N, Denmark2

Received 30 December 2002/ Returned for modification 26 March 2003/ Accepted 29 April 2003

Binding of immunoglobulin M (IgM) antibodies from normal human serum to the surface of Plasmodium falciparum-infected red blood cells (iRBC) has previously been demonstrated only in parasites that form rosettes with uninfected red cells. We show that natural, nonspecific IgM but not IgG, IgA, IgD, or IgE also binds to the surface of iRBC selected for adhesion to chondroitin sulfate A (CSA), a placental receptor for parasites associated with malaria in pregnancy. The protease sensitivity of IgM-binding appears to match that of CSA binding, suggesting that the two phenotypes may be mediated by the same parasite molecule. We also show that a wide range of mouse monoclonal antibodies of the IgM class bind nonspecifically to CSA-selected iRBC, an important consideration in the interpretation of immunological assays performed on these parasite lines.

* Corresponding author. Mailing address: Institute of Cell, Animal and Population Biology, University of Edinburgh, King's Buildings, West Mains Road, Edinburgh EH 9 3JT, United Kingdom. Phone: 44-131-650-8656. Fax: 44-131-650-6564. E-mail: acreasey{at}ed.ac.uk.

Editor: S. H. E. Kaufmann

Infection and Immunity, August 2003, p. 4767-4771, Vol. 71, No. 8
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.8.4767-4771.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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