This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Oñate, A. A. Articles by Andrews, E. Search for Related Content PubMed PubMed Citation Articles by Oñate, A. A. Articles by Andrews, E.

 Previous Article  |  Next Article 

Infection and Immunity, September 2003, p. 4857-4861, Vol. 71, No. 9
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.9.4857-4861.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

A DNA Vaccine Encoding Cu,Zn Superoxide Dismutase of Brucella abortus Induces Protective Immunity in BALB/c Mice

Molecular Immunology Laboratory, Department of Microbiology, Faculty of Biological Sciences, Universidad de Concepción, Concepción,¹ Department of Immunology, Faculty of Medicine, Universidad Austral, Valdivia, Chile²

Received 30 January 2003/ Returned for modification 7 May 2003/ Accepted 29 May 2003

This study was conducted to evaluate the immunogenicity and protective efficacy of a DNA vaccine encoding Brucella abortus Cu,Zn superoxide dismutase (SOD). Intramuscular injection of plasmid DNA carrying the SOD gene (pcDNA-SOD) into BALB/c mice elicited both humoral and cellular immune responses. Animals injected with pcDNA-SOD developed SOD-specific antibodies which exhibited a dominance of immunoglobulin G2a (IgG2a) over IgG1. In addition, the DNA vaccine elicited a T-cell-proliferative response and also induced the production of gamma interferon, but not interleukin-10 (IL-10) or IL-4, upon restimulation with either recombinant SOD or crude Brucella protein, suggesting the induction of a typical T-helper-1-dominated immune response in mice. The pcDNA-SOD (but not the control vector) induced a strong, significant level of protection in BALB/c mice against challenge with B. abortus virulent strain 2308; the level of protection was similar to the one induced by B. abortus vaccine strain RB51. Altogether, these data suggest that pcDNA-SOD is a good candidate for use in future studies of vaccination against brucellosis.

Editor: J. D. Clements

This article has been cited by other articles:

• Yang, X., Becker, T., Walters, N., Pascual, D. W. (2006). Deletion of znuA Virulence Factor Attenuates Brucella abortus and Confers Protection against Wild-Type Challenge. Infect. Immun. 74: 3874-3879 [Abstract] [Full Text]  
• Luo, D., Ni, B., Li, P., Shi, W., Zhang, S., Han, Y., Mao, L., He, Y., Wu, Y., Wang, X. (2006). Protective Immunity Elicited by a Divalent DNA Vaccine Encoding Both the L7/L12 and Omp16 Genes of Brucella abortus in BALB/c Mice.. Infect. Immun. 74: 2734-2741 [Abstract] [Full Text]  
• Yang, X., Hudson, M., Walters, N., Bargatze, R. F., Pascual, D. W. (2005). Selection of Protective Epitopes for Brucella melitensis by DNA Vaccination. Infect. Immun. 73: 7297-7303 [Abstract] [Full Text]  
• Cassataro, J., Velikovsky, C. A., de la Barrera, S., Estein, S. M., Bruno, L., Bowden, R., Pasquevich, K. A., Fossati, C. A., Giambartolomei, G. H. (2005). A DNA Vaccine Coding for the Brucella Outer Membrane Protein 31 Confers Protection against B. melitensis and B. ovis Infection by Eliciting a Specific Cytotoxic Response. Infect. Immun. 73: 6537-6546 [Abstract] [Full Text]  
• Onate, A. A., Donoso, G., Moraga-Cid, G., Folch, H., Cespedes, S., Andrews, E. (2005). An RNA Vaccine Based on Recombinant Semliki Forest Virus Particles Expressing the Cu,Zn Superoxide Dismutase Protein of Brucella abortus Induces Protective Immunity in BALB/c Mice. Infect. Immun. 73: 3294-3300 [Abstract] [Full Text]  
• Munoz-Montesino, C., Andrews, E., Rivers, R., Gonzalez-Smith, A., Moraga-Cid, G., Folch, H., Cespedes, S., Onate, A. A. (2004). Intraspleen Delivery of a DNA Vaccine Coding for Superoxide Dismutase (SOD) of Brucella abortus Induces SOD-Specific CD4+ and CD8+ T Cells. Infect. Immun. 72: 2081-2087 [Abstract] [Full Text]