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Infection and Immunity, September 2003, p. 5314-5323, Vol. 71, No. 9
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.9.5314-5323.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Mice Intranasally Immunized with a Recombinant 16-Kilodalton Antigen from Roundworm Ascaris Parasites Are Protected against Larval Migration of Ascaris suum

Naotoshi Tsuji,1* Kayo Suzuki,1 Harue Kasuga-Aoki,1 Takashi Isobe,1 Takeshi Arakawa,2 and Yasunobu Matsumoto3

Laboratory of Parasitic Diseases, National Institute of Animal Health, National Agricultural Research Organization, Tsukuba, Ibaraki 305-0856,1 Division of Molecular Microbiology, Center of Molecular Biosciences, University of the Ryukyus, Nishihara, Okinawa 903-0215,2 Laboratory of Global Animal Resource Science, Graduate School of Agricultural Life Science, University of Tokyo, Yayoi, Bunkyo, Tokyo 113-8657, Japan3

Received 10 March 2003/ Returned for modification 29 April 2003/ Accepted 18 June 2003

Protective immunity to the pig roundworm, Ascaris suum, has been demonstrated by immunization of pigs with antigens derived from the parasite's larval stages. We identified a protective antigen commonly expressed in the human and pig Ascaris infections as a 16-kDa protein (As16), which has no similarity at the amino acid level to mammalian proteins but has some similarity to those of the filarial parasites and Caenorhabditis elegans gene product. Localization analysis revealed that the native As16 was highly expressed in the adult worm intestine, hypodermis, and cuticles. In addition, As16 was detected in the parasite excretory and secretory products. Mice intranasally vaccinated with Escherichia coli-expressed recombinant As16 (rAs16), coupled with cholera toxin B subunit, generated a significant increase in the level of rAs16-specific immunoglobulin G (IgG) and IgE in serum. Mucosal IgA levels were also increased. The recombinant protein evoked a mixed (both Th1 and Th2) type of immune response characterized by elevated levels of gamma interferon and interleukin-10 in the culture supernatants of activated spleen cells. An increased level of IgG1 and IgG2a in serum was also observed. The vaccinated mice showed a reduction by 58% in the recovery of challenged larvae compared to a nonvaccinated control. These results suggest the possibility of developing a mucosal vaccine for human and pig ascariasis.

* Corresponding author. Mailing address: Laboratory of Parasitic Diseases, National Institute of Animal Health, National Agricultural Research Organization, 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856 Japan. Phone: 81-29-838-7749. Fax: 81-29-838-7880. E-mail: tsujin{at}affrc.go.jp.

Editor: J. M. Mansfield

Infection and Immunity, September 2003, p. 5314-5323, Vol. 71, No. 9
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.9.5314-5323.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Islam, M. K., Miyoshi, T., Yamada, M., Tsuji, N. (2005). Pyrophosphatase of the Roundworm Ascaris suum Plays an Essential Role in the Worm's Molting and Development. Infect. Immun. 73: 1995-2004 [Abstract] [Full Text]  
  • Bungiro, R. D. Jr., Solis, C. V., Harrison, L. M., Cappello, M. (2004). Purification and Molecular Cloning of and Immunization with Ancylostoma ceylanicum Excretory-Secretory Protein 2, an Immunoreactive Protein Produced by Adult Hookworms. Infect. Immun. 72: 2203-2213 [Abstract] [Full Text]  


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