This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Harper, M.
Right arrow Articles by Adler, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Harper, M.
Right arrow Articles by Adler, B.

 Previous Article

Infection and Immunity, September 2003, p. 5440-5446, Vol. 71, No. 9
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.9.5440-5446.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Signature-Tagged Mutagenesis of Pasteurella multocida Identifies Mutants Displaying Differential Virulence Characteristics in Mice and Chickens

Marina Harper,1 John D. Boyce,1 Ian W. Wilkie,2 and Ben Adler1*

Bacterial Pathogenesis Research Group, Department of Microbiology, Monash University, Clayton, Victoria 3800,1 Veterinary Pathology and Anatomy, University of Queensland, Brisbane, Queensland 4072, Australia2

Received 14 April 2003/ Returned for modification 30 May 2003/ Accepted 16 June 2003

Pasteurella multocida is the causative agent of fowl cholera in birds. Signature-tagged mutagenesis (STM) was used to identify potential virulence factors in a mouse septicemia disease model and a chicken fowl cholera model. A library of P. multocida mutants was constructed with a modified Tn916 and screened for attenuation in both animal models. Mutants identified by the STM screening were confirmed as attenuated by competitive growth assays in both chickens and mice. Of the 15 mutants identified in the chicken model, only 5 were also attenuated in mice, showing for the first time the presence of host-specific virulence factors and indicating the importance of screening for attenuation in the natural host.

* Corresponding author. Mailing address: Bacterial Pathogenesis Research Group, Department of Microbiology, Monash University, Clayton, Victoria 3800, Australia. Phone: 61 03 9905 4815. Fax: 61 03 9905 4811. E-mail: Ben.Adler{at}med.monash.edu.au.

Editor: J. T. Barbieri

Infection and Immunity, September 2003, p. 5440-5446, Vol. 71, No. 9
0019-9567/03/$08.00+0     DOI: 10.1128/IAI.71.9.5440-5446.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Boyce, J. D., Harper, M., St. Michael, F., John, M., Aubry, A., Parnas, H., Logan, S. M., Wilkie, I. W., Ford, M., Cox, A. D., Adler, B. (2009). Identification of Novel Glycosyltransferases Required for Assembly of the Pasteurella multocida A:1 Lipopolysaccharide and Their Involvement in Virulence. Infect. Immun. 77: 1532-1542 [Abstract] [Full Text]  
  • Hatfaludi, T., Al-Hasani, K., Dunstone, M., Boyce, J., Adler, B. (2008). Characterization of TolC Efflux Pump Proteins from Pasteurella multocida. Antimicrob. Agents Chemother. 52: 4166-4171 [Abstract] [Full Text]  
  • Derouaux, A., Wolf, B., Fraipont, C., Breukink, E., Nguyen-Disteche, M., Terrak, M. (2008). The Monofunctional Glycosyltransferase of Escherichia coli Localizes to the Cell Division Site and Interacts with Penicillin-Binding Protein 3, FtsW, and FtsN. J. Bacteriol. 190: 1831-1834 [Abstract] [Full Text]  
  • Harper, M., Boyce, J. D., Cox, A. D., St. Michael, F., Wilkie, I. W., Blackall, P. J., Adler, B. (2007). Pasteurella multocida Expresses Two Lipopolysaccharide Glycoforms Simultaneously, but Only a Single Form Is Required for Virulence: Identification of Two Acceptor-Specific Heptosyl I Transferases. Infect. Immun. 75: 3885-3893 [Abstract] [Full Text]  
  • Perez, B. A., Planet, P. J., Kachlany, S. C., Tomich, M., Fine, D. H., Figurski, D. H. (2006). Genetic Analysis of the Requirement for flp-2, tadV, and rcpB in Actinobacillus actinomycetemcomitans Biofilm Formation.. J. Bacteriol. 188: 6361-6375 [Abstract] [Full Text]  
  • Terrak, M., Nguyen-Disteche, M. (2006). Kinetic Characterization of the Monofunctional Glycosyltransferase from Staphylococcus aureus.. J. Bacteriol. 188: 2528-2532 [Abstract] [Full Text]  
  • Ku, Y.-W., McDonough, S. P., Palaniappan, R. U. M., Chang, C.-F., Chang, Y.-F. (2005). Novel Attenuated Salmonella enterica Serovar Choleraesuis Strains as Live Vaccine Candidates Generated by Signature-Tagged Mutagenesis. Infect. Immun. 73: 8194-8203 [Abstract] [Full Text]  
  • Ojha, S., Sirois, M., MacInnes, J. I. (2005). Identification of Actinobacillus suis Genes Essential for the Colonization of the Upper Respiratory Tract of Swine. Infect. Immun. 73: 7032-7039 [Abstract] [Full Text]  
  • Hundt, M. J., Ruffolo, C. G. (2005). Interaction of Pasteurella multocida with Free-Living Amoebae. Appl. Environ. Microbiol. 71: 5458-5464 [Abstract] [Full Text]  
  • Boucher, D. J., Adler, B., Boyce, J. D. (2005). The Pasteurella multocida nrfE Gene Is Upregulated during Infection and Is Essential for Nitrite Reduction but Not for Virulence. J. Bacteriol. 187: 2278-2285 [Abstract] [Full Text]  
  • Steenbergen, S. M., Lichtensteiger, C. A., Caughlan, R., Garfinkle, J., Fuller, T. E., Vimr, E. R. (2005). Sialic Acid Metabolism and Systemic Pasteurellosis. Infect. Immun. 73: 1284-1294 [Abstract] [Full Text]  
  • Harper, M., Cox, A. D., St. Michael, F., Wilkie, I. W., Boyce, J. D., Adler, B. (2004). A Heptosyltransferase Mutant of Pasteurella multocida Produces a Truncated Lipopolysaccharide Structure and Is Attenuated in Virulence. Infect. Immun. 72: 3436-3443 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»