This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ren, B.
Right arrow Articles by Briles, D. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ren, B.
Right arrow Articles by Briles, D. E.

 Previous Article  |  Next Article 

Infection and Immunity, January 2004, p. 114-122, Vol. 72, No. 1
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.1.114-122.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Effects of PspA and Antibodies to PspA on Activation and Deposition of Complement on the Pneumococcal Surface

Bing Ren,1* Alexander J. Szalai,2 Susan K. Hollingshead,1 and David E. Briles1

Department of Microbiology,1 Division of Clinical Immunology and Rheumatology, The University of Alabama at Birmingham, Birmingham, Alabama, 352942

Received 11 August 2003/ Returned for modification 15 September 2003/ Accepted 14 October 2003

Streptococcus pneumoniae infection is a frequent cause of pneumonia, otitis media, meningitis, and septicemia. Pneumococcal surface protein A (PspA) is an important virulence factor on the pathogen surface, and it is known to interfere with complement activation. In this study, flow cytometry was used to study the effects of PspA and antibodies to PspA on the deposition of complement C3 on the surface of a capsular type 3 strain, WU2, and its PspA- mutant, JY1119. Using naive mouse serum as a complement source, measurable deposition of C3 was observed within 4 min on PspA- pneumococci, and the amount of surface-bound C3 accumulated rapidly as the amount of serum was increased. In contrast, very little C3 was deposited on the PspA+ strain. In nonimmune mouse serum, the classical pathway was the dominant activation pathway triggered by PspA- pneumococci. Accordingly, EGTA blocked almost all of the complement activation. Moreover, a significant amount of C3 was still deposited on the PspA- strain when serum from factor B-deficient mice was used. This deposition was not observed on the PspA+ pneumococci, indicating that PspA may inhibit complement deposition via the classical pathway. Furthermore, under the conditions we tested, PspA also inhibited C3 deposition when the classical pathway was initiated by antibodies to capsular polysaccharide. Antibodies to PspA could overcome the anticomplementary effect of PspA, allowing for increased complement activation and C3 deposition onto PspA+ bacteria.

* Corresponding author. Mailing address: 845 19th St. S., BBRB 658, Box 10, Birmingham, AL 35294. Phone: (205) 934-1880. Fax: (205) 934-0605. E-mail: bing_ren{at}microbio.uab.edu.

Editor: D. L. Burns

Infection and Immunity, January 2004, p. 114-122, Vol. 72, No. 1
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.1.114-122.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Xin, W., Li, Y., Mo, H., Roland, K. L., Curtiss, R. III (2009). PspA Family Fusion Proteins Delivered by Attenuated Salmonella enterica Serovar Typhimurium Extend and Enhance Protection against Streptococcus pneumoniae. Infect. Immun. 77: 4518-4528 [Abstract] [Full Text]  
  • Schachern, P. A., Tsuprun, V., Cureoglu, S., Ferrieri, P., Briles, D. E., Paparella, M. M., Juhn, S. (2009). Virulence of Pneumococcal Proteins on the Inner Ear. Arch Otolaryngol Head Neck Surg 135: 657-661 [Abstract] [Full Text]  
  • Rohatgi, S., Dutta, D., Tahir, S., Sehgal, D. (2009). Molecular Dissection of Antibody Responses against Pneumococcal Surface Protein A: Evidence for Diverse DH-Less Heavy Chain Gene Usage and Avidity Maturation. J. Immunol. 182: 5570-5585 [Abstract] [Full Text]  
  • Tian, H., Weber, S., Thorkildson, P., Kozel, T. R., Pirofski, L.-a. (2009). Efficacy of Opsonic and Nonopsonic Serotype 3 Pneumococcal Capsular Polysaccharide-Specific Monoclonal Antibodies against Intranasal Challenge with Streptococcus pneumoniae in Mice. Infect. Immun. 77: 1502-1513 [Abstract] [Full Text]  
  • Sabharwal, V., Ram, S., Figueira, M., Park, I. H., Pelton, S. I. (2009). Role of Complement in Host Defense against Pneumococcal Otitis Media. Infect. Immun. 77: 1121-1127 [Abstract] [Full Text]  
  • Li, J., Szalai, A. J., Hollingshead, S. K., Nahm, M. H., Briles, D. E. (2009). Antibody to the Type 3 Capsule Facilitates Immune Adherence of Pneumococci to Erythrocytes and Augments Their Transfer to Macrophages. Infect. Immun. 77: 464-471 [Abstract] [Full Text]  
  • Lu, L., Ma, Z., Jokiranta, T. S., Whitney, A. R., DeLeo, F. R., Zhang, J.-R. (2008). Species-Specific Interaction of Streptococcus pneumoniae with Human Complement Factor H. J. Immunol. 181: 7138-7146 [Abstract] [Full Text]  
  • Melin, M. M., Hollingshead, S. K., Briles, D. E., Lahdenkari, M. I., Kilpi, T. M., Kayhty, H. M. (2008). Development of Antibodies to PspA Families 1 and 2 in Children after Exposure to Streptococcus pneumoniae. CVI 15: 1529-1535 [Abstract] [Full Text]  
  • Schachern, P., Tsuprun, V., Cureoglu, S., Ferrieri, P., Briles, D., Paparella, M., Juhn, S. (2008). The Round Window Membrane in Otitis Media: Effect of Pneumococcal Proteins. Arch Otolaryngol Head Neck Surg 134: 658-662 [Abstract] [Full Text]  
  • Ferreira, D. M., Darrieux, M., Oliveira, M. L. S., Leite, L. C. C., Miyaji, E. N. (2008). Optimized Immune Response Elicited by a DNA Vaccine Expressing Pneumococcal Surface Protein A Is Characterized by a Balanced Immunoglobulin G1 (IgG1)/IgG2a Ratio and Proinflammatory Cytokine Production. CVI 15: 499-505 [Abstract] [Full Text]  
  • Li, J., Glover, D. T., Szalai, A. J., Hollingshead, S. K., Briles, D. E. (2007). PspA and PspC Minimize Immune Adherence and Transfer of Pneumococci from Erythrocytes to Macrophages through Their Effects on Complement Activation. Infect. Immun. 75: 5877-5885 [Abstract] [Full Text]  
  • Darrieux, M., Miyaji, E. N., Ferreira, D. M., Lopes, L. M., Lopes, A. P. Y., Ren, B., Briles, D. E., Hollingshead, S. K., Leite, L. C. C. (2007). Fusion Proteins Containing Family 1 and Family 2 PspA Fragments Elicit Protection against Streptococcus pneumoniae That Correlates with Antibody-Mediated Enhancement of Complement Deposition. Infect. Immun. 75: 5930-5938 [Abstract] [Full Text]  
  • Tian, H., Groner, A., Boes, M., Pirofski, L.-a. (2007). Pneumococcal Capsular Polysaccharide Vaccine-Mediated Protection against Serotype 3 Streptococcus pneumoniae in Immunodeficient Mice. Infect. Immun. 75: 1643-1650 [Abstract] [Full Text]  
  • Quin, L. R., Moore, Q. C. III, McDaniel, L. S. (2007). Pneumolysin, PspA, and PspC Contribute to Pneumococcal Evasion of Early Innate Immune Responses during Bacteremia in Mice. Infect. Immun. 75: 2067-2070 [Abstract] [Full Text]  
  • Burton, R. L., Nahm, M. H. (2006). Development and Validation of a Fourfold Multiplexed Opsonization Assay (MOPA4) for Pneumococcal Antibodies.. CVI 13: 1004-1009 [Abstract] [Full Text]  
  • Ferreira, D. M., Miyaji, E. N., Oliveira, M. L. S., Darrieux, M., Areas, A. P. M., Ho, P. L., Leite, L. C. C. (2006). DNA vaccines expressing pneumococcal surface protein A (PspA) elicit protection levels comparable to recombinant protein.. J Med Microbiol 55: 375-378 [Abstract] [Full Text]  
  • Hollingshead, S. K., Baril, L., Ferro, S., King, J., Coan, P., Briles, D. E., the Pneumococcal Proteins Epi Study Group, (2006). Pneumococcal surface protein A (PspA) family distribution among clinical isolates from adults over 50 years of age collected in seven countries. J Med Microbiol 55: 215-221 [Abstract] [Full Text]  
  • Ng, W.-L., Tsui, H.-C. T., Winkler, M. E. (2005). Regulation of the pspA Virulence Factor and Essential pcsB Murein Biosynthetic Genes by the Phosphorylated VicR (YycF) Response Regulator in Streptococcus pneumoniae. J. Bacteriol. 187: 7444-7459 [Abstract] [Full Text]  
  • Briles, D. E., Novak, L., Hotomi, M., van Ginkel, F. W., King, J. (2005). Nasal Colonization with Streptococcus pneumoniae Includes Subpopulations of Surface and Invasive Pneumococci. Infect. Immun. 73: 6945-6951 [Abstract] [Full Text]  
  • Nordstrom, T., Blom, A. M., Tan, T. T., Forsgren, A., Riesbeck, K. (2005). Ionic Binding of C3 to the Human Pathogen Moraxella catarrhalis Is a Unique Mechanism for Combating Innate Immunity. J. Immunol. 175: 3628-3636 [Abstract] [Full Text]  
  • Yuste, J., Botto, M., Paton, J. C., Holden, D. W., Brown, J. S. (2005). Additive Inhibition of Complement Deposition by Pneumolysin and PspA Facilitates Streptococcus pneumoniae Septicemia. J. Immunol. 175: 1813-1819 [Abstract] [Full Text]  
  • Kerr, A. R., Paterson, G. K., Riboldi-Tunnicliffe, A., Mitchell, T. J. (2005). Innate Immune Defense against Pneumococcal Pneumonia Requires Pulmonary Complement Component C3. Infect. Immun. 73: 4245-4252 [Abstract] [Full Text]  
  • Areas, A. P. M., Oliveira, M. L. S., Miyaji, E. N., Leite, L. C. C., Ho, P. L. (2005). Intradermal Immunization of Mice with Cholera Toxin B-Pneumococcal Surface Protein A Fusion Protein Is Protective against Intraperitoneal Challenge with Streptococcus pneumoniae. Infect. Immun. 73: 3810-3813 [Full Text]  
  • Gor, D. O., Ding, X., Briles, D. E., Jacobs, M. R., Greenspan, N. S. (2005). Relationship between Surface Accessibility for PpmA, PsaA, and PspA and Antibody-Mediated Immunity to Systemic Infection by Streptococcus pneumoniae. Infect. Immun. 73: 1304-1312 [Abstract] [Full Text]  
  • Ren, B., McCrory, M. A., Pass, C., Bullard, D. C., Ballantyne, C. M., Xu, Y., Briles, D. E., Szalai, A. J. (2004). The Virulence Function of Streptococcus pneumoniae Surface Protein A Involves Inhibition of Complement Activation and Impairment of Complement Receptor-Mediated Protection. J. Immunol. 173: 7506-7512 [Abstract] [Full Text]  
  • Shaper, M., Hollingshead, S. K., Benjamin, W. H. Jr., Briles, D. E. (2004). PspA Protects Streptococcus pneumoniae from Killing by Apolactoferrin, and Antibody to PspA Enhances Killing of Pneumococci by Apolactoferrin. Infect. Immun. 72: 5031-5040 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»