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Infection and Immunity, January 2004, p. 598-601, Vol. 72, No. 1
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.1.598-601.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Relationship between Candida albicans Virulence during Experimental Hematogenously Disseminated Infection and Endothelial Cell Damage In Vitro

Angela A. Sanchez,1 Douglas A. Johnston,1 Carter Myers,1 John E. Edwards Jr.,1,2 Aaron P. Mitchell,3 and Scott G. Filler1,2*

St. Johns Cardiovascular Research Center, Division of Infectious Diseases, Department of Medicine, Harbor-UCLA Research and Education Institute, Torrance, California 90502,1 The David Geffen School of Medicine at UCLA, Los Angeles, California 90024,2 Department of Microbiology and Integrated Program in Cellular, Molecular, and Biophysical Studies, Columbia University, New York, New York 100323

Received 4 September 2003/ Returned for modification 15 September 2003/ Accepted 22 September 2003

Candida albicans must penetrate the endothelial cell lining of the vasculature to invade the deep tissues during a hematogenously disseminated infection. We compared 27 C. albicans mutants with their wild-type parent for their capacity to damage endothelial cells in vitro and cause a lethal infection in mice following tail vein inoculation. Of 10 mutants with significantly impaired capacity to damage endothelial cells, all had attenuated virulence. Therefore, the endothelial cell damage assay can be used as a screen to identify some virulence factors relevant to hematogenously disseminated candidiasis.

* Corresponding author. Mailing address: Division of Infectious Diseases, Harbor-UCLA Research and Education Institute, 1124 W. Carson St., Torrance, CA 90502. Phone: (310) 222-6426. Fax: (310) 782-2016. E-mail: sfiller{at}ucla.edu.

Editor: T. R. Kozel

Infection and Immunity, January 2004, p. 598-601, Vol. 72, No. 1
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.1.598-601.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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