This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sanchez, A. A.
Right arrow Articles by Filler, S. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sanchez, A. A.
Right arrow Articles by Filler, S. G.

 Previous Article  |  Next Article 

Infection and Immunity, January 2004, p. 598-601, Vol. 72, No. 1
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.1.598-601.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Relationship between Candida albicans Virulence during Experimental Hematogenously Disseminated Infection and Endothelial Cell Damage In Vitro

Angela A. Sanchez,1 Douglas A. Johnston,1 Carter Myers,1 John E. Edwards Jr.,1,2 Aaron P. Mitchell,3 and Scott G. Filler1,2*

St. Johns Cardiovascular Research Center, Division of Infectious Diseases, Department of Medicine, Harbor-UCLA Research and Education Institute, Torrance, California 90502,1 The David Geffen School of Medicine at UCLA, Los Angeles, California 90024,2 Department of Microbiology and Integrated Program in Cellular, Molecular, and Biophysical Studies, Columbia University, New York, New York 100323

Received 4 September 2003/ Returned for modification 15 September 2003/ Accepted 22 September 2003

Candida albicans must penetrate the endothelial cell lining of the vasculature to invade the deep tissues during a hematogenously disseminated infection. We compared 27 C. albicans mutants with their wild-type parent for their capacity to damage endothelial cells in vitro and cause a lethal infection in mice following tail vein inoculation. Of 10 mutants with significantly impaired capacity to damage endothelial cells, all had attenuated virulence. Therefore, the endothelial cell damage assay can be used as a screen to identify some virulence factors relevant to hematogenously disseminated candidiasis.


* Corresponding author. Mailing address: Division of Infectious Diseases, Harbor-UCLA Research and Education Institute, 1124 W. Carson St., Torrance, CA 90502. Phone: (310) 222-6426. Fax: (310) 782-2016. E-mail: sfiller{at}ucla.edu.

Editor: T. R. Kozel


Infection and Immunity, January 2004, p. 598-601, Vol. 72, No. 1
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.1.598-601.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.




This article has been cited by other articles:

  • Park, H., Liu, Y., Solis, N., Spotkov, J., Hamaker, J., Blankenship, J. R., Yeaman, M. R., Mitchell, A. P., Liu, H., Filler, S. G. (2009). Transcriptional Responses of Candida albicans to Epithelial and Endothelial Cells. Eukaryot Cell 8: 1498-1510 [Abstract] [Full Text]  
  • Grubb, S. E. W., Murdoch, C., Sudbery, P. E., Saville, S. P., Lopez-Ribot, J. L., Thornhill, M. H. (2009). Adhesion of Candida albicans to Endothelial Cells under Physiological Conditions of Flow. Infect. Immun. 77: 3872-3878 [Abstract] [Full Text]  
  • Sabina, J., Brown, V. (2009). Glucose Sensing Network in Candida albicans: a Sweet Spot for Fungal Morphogenesis. Eukaryot Cell 8: 1314-1320 [Full Text]  
  • Canaud, G., Timsit, M.-O., Zuber, J., Bougnoux, M.-E., Mejean, A., Thervet, E., Snanoudj, R., Sberro, R., Martinez, F., Legendre, C., Mamzer-Bruneel, M.-F. (2009). Early conservative intervention for candida contamination of preservative fluid without allograft nephrectomy. Nephrol Dial Transplant 24: 1325-1327 [Abstract] [Full Text]  
  • Grubb, S. E. W., Murdoch, C., Sudbery, P. E., Saville, S. P., Lopez-Ribot, J. L., Thornhill, M. H. (2008). Candida albicans-Endothelial Cell Interactions: a Key Step in the Pathogenesis of Systemic Candidiasis. Infect. Immun. 76: 4370-4377 [Full Text]  
  • Gank, K. D., Yeaman, M. R., Kojima, S., Yount, N. Y., Park, H., Edwards, J. E. Jr., Filler, S. G., Fu, Y. (2008). SSD1 Is Integral to Host Defense Peptide Resistance in Candida albicans. Eukaryot Cell 7: 1318-1327 [Abstract] [Full Text]  
  • Brand, A., Vacharaksa, A., Bendel, C., Norton, J., Haynes, P., Henry-Stanley, M., Wells, C., Ross, K., Gow, N. A. R., Gale, C. A. (2008). An Internal Polarity Landmark Is Important for Externally Induced Hyphal Behaviors in Candida albicans. Eukaryot Cell 7: 712-720 [Abstract] [Full Text]  
  • Norice, C. T., Smith, F. J. Jr., Solis, N., Filler, S. G., Mitchell, A. P. (2007). Requirement for Candida albicans Sun41 in Biofilm Formation and Virulence. Eukaryot Cell 6: 2046-2055 [Abstract] [Full Text]  
  • Nobile, C. J., Nett, J. E., Andes, D. R., Mitchell, A. P. (2006). Function of Candida albicans Adhesin Hwp1 in Biofilm Formation.. Eukaryot Cell 5: 1604-1610 [Abstract] [Full Text]  
  • Martinez-Lopez, R., Park, H., Myers, C. L., Gil, C., Filler, S. G. (2006). Candida albicans Ecm33p Is Important for Normal Cell Wall Architecture and Interactions with Host Cells. Eukaryot Cell 5: 140-147 [Abstract] [Full Text]  
  • Rouabhia, M., Schaller, M., Corbucci, C., Vecchiarelli, A., Prill, S. K.-H., Giasson, L., Ernst, J. F. (2005). Virulence of the Fungal Pathogen Candida albicans Requires the Five Isoforms of Protein Mannosyltransferases. Infect. Immun. 73: 4571-4580 [Abstract] [Full Text]  
  • Villar, C. C., Kashleva, H., Mitchell, A. P., Dongari-Bagtzoglou, A. (2005). Invasive Phenotype of Candida albicans Affects the Host Proinflammatory Response to Infection. Infect. Immun. 73: 4588-4595 [Abstract] [Full Text]  
  • Toenjes, K. A., Munsee, S. M., Ibrahim, A. S., Jeffrey, R., Edwards, J. E. Jr., Johnson, D. I. (2005). Small-Molecule Inhibitors of the Budded-to-Hyphal-Form Transition in the Pathogenic Yeast Candida albicans. Antimicrob. Agents Chemother. 49: 963-972 [Abstract] [Full Text]