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Infection and Immunity, January 2004, p. 615-620, Vol. 72, No. 1
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.1.615-620.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Antibody-Mediated Neutralization of Pertussis Toxin-Induced Mitogenicity of Human Peripheral Blood Mononuclear Cells

Scott H. Millen,1 David I. Bernstein,2 Beverly Connelly,2 Joel I. Ward,3 Swei-Ju Chang,3 and Alison A. Weiss1*

Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati, Cincinnati, Ohio 45267,1 Division of Infectious Diseases, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio 45229,2 UCLA Center for Vaccine Research, Harbor-UCLA Medical Center, Torrance, California 905023

Received 8 July 2003/ Returned for modification 12 September 2003/ Accepted 17 September 2003

Antibody-mediated neutralization of pertussis toxin-induced proliferation of human peripheral blood mononuclear cells (PBMC) was assessed using alamarBlue and compared with results from the Chinese hamster ovary (CHO) cell assay using sera from vaccinated adults and convalescent children. Neutralization values for the CHO assay were similar for vaccinated and convalescent subjects; however. the convalescent group had higher titers in the PBMC assay. Results for pertussis toxin neutralization with the CHO assay appear to be distinct from those with the PBMC assay.

* Corresponding author. Mailing address: Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati, 231 Albert Sabin Way, Cincinnati, OH 45267-0524. Phone: (513) 558-2820. Fax: (513) 558-8474. E-mail: alison.weiss{at}uc.edu.

Editor: J. T. Barbieri

Infection and Immunity, January 2004, p. 615-620, Vol. 72, No. 1
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.1.615-620.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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  • Weiss, A. A., Patton, A. K., Millen, S. H., Chang, S.-J., Ward, J. I., Bernstein, D. I. (2004). Acellular Pertussis Vaccines and Complement Killing of Bordetella pertussis. Infect. Immun. 72: 7346-7351 [Abstract] [Full Text]  


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