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Infection and Immunity, October 2004, p. 5548-5554, Vol. 72, No. 10
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.10.5548-5554.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Functional Analysis of the Tsh Autotransporter from an Avian Pathogenic Escherichia coli Strain

Maria Kostakioti and Christos Stathopoulos^*

Department of Biology and Biochemistry, University of Houston, Houston, Texas

Received 13 January 2004/ Returned for modification 10 February 2004/ Accepted 22 June 2004

The temperature-sensitive hemagglutinin (Tsh) is an autotransporter protein secreted by avian-pathogenic Escherichia coli strains that colonize the respiratory tract and lead to airsacculitis, pericarditis, and colisepticemia. It is synthesized as a 140-kDa precursor protein, whose processing results in a 106-kDa passenger domain (Tsh_s) and a 33-kDa ß-domain (Tsh_ß). The presence of a conserved 7-amino-acid serine protease motif within Tsh_s classifies the protein in a subfamily of autotransporters, known as serine protease autotransporters of the Enterobacteriaceae. In this study, we report that purified Tsh_s is capable of adhering to red blood cells, hemoglobin, and the extracellular matrix proteins fibronectin and collagen IV. We also demonstrate that Tsh_s exerts proteolytic activity against casein, and we provide experimental evidence demonstrating that serine 259 is essential for the protease function. However, this residue is not required for adherence to substrates, and its replacement by an alanine does not abolish binding activity. In summary, our results demonstrate that Tsh is a bifunctional protein with both adhesive and proteolytic properties.

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* Corresponding author. Mailing address: Department of Biology and Biochemistry, SRII 369, University of Houston, 4800 Calhoun St., Houston, TX 77204. Phone: (713) 743-2491. Fax: (713) 743-8351. E-mail: cstatho{at}uh.edu.

Editor: J. B. Bliska

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Infection and Immunity, October 2004, p. 5548-5554, Vol. 72, No. 10
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.10.5548-5554.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

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