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Infection and Immunity, October 2004, p. 5791-5798, Vol. 72, No. 10
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.10.5791-5798.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Reduced Expression of Gamma Interferon in Serum and Marked Lymphoid Depletion Induced by Porphyromonas gingivalis Increase Murine Morbidity and Mortality due to Cytomegalovirus Infection

Jacob Stern,^1, Ela Shai,^1, Batia Zaks,¹ Amal Halabi,² Yael Houri-Haddad,² Lior Shapira,² and Aaron Palmon^1*

Institute of Dental Sciences,¹ Department of Periodontology Faculty of Dental Medicine, Hebrew University-Hadassah, Jerusalem Israel²

Received 24 March 2004/ Returned for modification 2 June 2004/ Accepted 30 June 2004

Porphyromonas gingivalis, a gram-negative anaerobe, is a major etiological agent of severe forms of periodontal disease. Although periodontal disease is considered a localized disease, accumulating evidence indicates that it may lead to a predisposition to a decline in immunocompetence. Human cytomegalovirus (CMV) commonly infects all human populations without producing significant clinical symptoms. Immunocompromised patients usually develop a primary or reactivated CMV infection, which is associated with high rates of morbidity and mortality. The aim of this study was to determine whether P. gingivalis increases animal susceptibility to CMV infection. Mice were inoculated with CMV and infected locally with P. gingivalis 3 days after the virus inoculation. Mortality rates were monitored, and traces of viral DNA and bacterial infection were detected systemically by using real-time PCR. Local and systemic cytokine secretion was measured, and histological sections were used to assess the pathological state of infected organs. P. gingivalis- and CMV-coinfected mice showed dramatically higher mortality rates than mice infected with P. gingivalis or CMV only. Although the organs of coinfected mice exhibited decreased viral titers, distinct necrosis and tissue damage were more evident in the livers and spleens of these mice than in those of mice infected with CMV only. Furthermore, systemic gamma interferon levels were decreased in coinfected mice, and marked lymphoid depletion was observed in their necrotic organs. In parallel control Escherichia coli-CMV coinfection experiments, the mortality and pathological results were the same as those found in mice infected with CMV only. Our results suggest a specific influence of P. gingivalis on the mouse immune response, causing increased susceptibility to CMV infection.

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* Corresponding author. Mailing address: Institute of Dental Sciences, Faculty of Dental Medicine, Hebrew University-Hadassah, Jerusalem, Israel. Phone: 972-2-6757595. Fax: 972-2-6757595. E-mail: kdela{at}cc.huji.ac.il.

Editor: F. C. Fang

J.S. and E.S. contributed equally to this work.

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Infection and Immunity, October 2004, p. 5791-5798, Vol. 72, No. 10
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.10.5791-5798.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.