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Infection and Immunity, October 2004, p. 5963-5971, Vol. 72, No. 10
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.10.5963-5971.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Magnetic Resonance Imaging of Pulmonary Lesions in Guinea Pigs Infected with Mycobacterium tuberculosis

Susan L. Kraft,1 Deanna Dailey,1 Matthew Kovach,1 Karen L. Stasiak,2 Jamie Bennett,2 Christine T. McFarland,3 David N. McMurray,3 Angelo A. Izzo,2 Ian M. Orme,2 and Randall J. Basaraba2*

Departments of Environmental and Radiological Health Sciences,1 Microbiology, Immunology, and Pathology Colorado State University, Fort Collins, Colorado,2 Department of Medical Microbiology and Immunology, Texas A&M University, College Station, Texas3

Received 1 March 2004/ Returned for modification 10 May 2004/ Accepted 21 June 2004

We utilized magnetic resonance imaging to visualize lesions in the lungs of guinea pigs infected by low-dose aerosol exposure to Mycobacterium tuberculosis. Lesions were prominent in such images, and colorized three-dimensional reconstructions of images revealed a very uniform distribution in the lungs. Lesion numbers after 1 month were approximately similar to the aerosol exposure algorithm, suggesting that each was established by a single bacterium. Numbers of lesions in unprotected and vaccinated animals were similar over the first month but increased thereafter in the control animals, indicating secondary lesion development. Whereas lesion sizes increased progressively in control guinea pigs, lesions remained small in BCG-vaccinated animals. A prominent feature of the disease pathology in unprotected animals was rapid and severe lymphadenopathy of the mediastinal lymph node cluster, which is paradoxical given the strong state of cellular immunity at this time. Further development of this technical approach could be very useful in tracking lesion size, number, and progression in the search for new tuberculosis vaccines.

* Corresponding author. Mailing address: Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523. Phone: (970) 491-3313. Fax: (970) 491-0603. E-mail: basaraba{at}colostate.edu.

Editor: S. H. E. Kaufmann

Infection and Immunity, October 2004, p. 5963-5971, Vol. 72, No. 10
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.10.5963-5971.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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