This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hashim, A.
Right arrow Articles by Bourke, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hashim, A.
Right arrow Articles by Bourke, B.

 Previous Article  |  Next Article 

Infection and Immunity, October 2004, p. 6125-6131, Vol. 72, No. 10
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.10.6125-6131.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Host Cell Tropism Underlies Species Restriction of Human and Bovine Cryptosporidium parvum Genotypes

Amna Hashim,1,2,3 Marguerite Clyne,1,2 Grace Mulcahy,3 Donna Akiyoshi,4 Rachel Chalmers,5 and Billy Bourke1,2*

The Children's Research Centre, Our Lady's Hospital for Sick Children, Crumlin,1 Departments of Paediatrics,2 Veterinary Microbiology and Parasitology Conway Institute for Biomolecular and Biomedical Research, University College, Dublin, Ireland,3 Division of Infectious Diseases, Tufts University School of Veterinary Medicine, North Grafton, Massachusetts,4 Cryptosporidium Reference Unit, NPHS Microbiology Swansea, Singleton Hospital, Swansea, Wales, United Kingdom5

Received 31 March 2004/ Returned for modification 22 May 2004/ Accepted 15 July 2004

It has been recognized recently that human cryptosporidiosis is usually caused by Cryptosporidium parvum genotype I ("human" C. parvum), which is not found in animals. Compared to C. parvum genotype II, little is known of the biology of invasion of the human-restricted C. parvum genotype I. The aims of the present study were (i) to explore and compare with genotype II the pathogenesis of C. parvum genotype I infection by using an established in vitro model of infection and (ii) to examine the possibility that host-specific cell tropism determines species restriction among C. parvum genotypes by using a novel ex vivo small intestinal primary cell model of infection. Oocysts of C. parvum genotypes I and II were used to infect HCT-8 cells and primary intestinal epithelial cells in vitro. Primary cells were harvested from human endoscopic small-bowel biopsies and from bovine duodenum postmortem. C. parvum genotype I infected HCT-8 cells with lower efficiency than C. parvum genotype II. Actin colocalization at the host parasite interface and reduction in levels of invasion after treatment with microfilament inhibitors (cytochalasin B and cytochalasin D) were observed for both genotypes. C. parvum genotype II invaded primary intestinal epithelial cells, regardless of the species of origin. In contrast, C. parvum genotype I invaded only human small-bowel cells. The pathogenesis of C. parvum genotype I differs from C. parvum genotype II. C parvum genotype I does not enter primary bovine intestinal cells, suggesting that the species restriction of this genotype is due to host tissue tropism of the infecting isolate.

* Corresponding author. Mailing address: Professorial Unit, The Children's Research Centre, Our Lady's Hospital for Sick Children, Crumlin, Dublin 12, Ireland. Phone: 35314556901. Fax: 35314555307. E-mail: billy.bourke{at}ucd.ie.

Editor: W. A. Petri, Jr.

Infection and Immunity, October 2004, p. 6125-6131, Vol. 72, No. 10
0019-9567/04/$08.00+0     DOI: 10.1128/IAI.72.10.6125-6131.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Byrne, C. M., Clyne, M., Bourke, B. (2007). Campylobacter jejuni adhere to and invade chicken intestinal epithelial cells in vitro. Microbiology 153: 561-569 [Abstract] [Full Text]  
  • Hashim, A., Mulcahy, G., Bourke, B., Clyne, M. (2006). Interaction of Cryptosporidium hominis and Cryptosporidium parvum with Primary Human and Bovine Intestinal Cells. Infect. Immun. 74: 99-107 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»